A non sense suppression is a suppression which is due to stop codon i.e,s codon get mutated and resulted in stop codon and truncated protein will produce then a nonsense suppressor will play the role and will help the cell to produced the substituted amino acids.A nonsense suppressor is a tRNA mutation that suppresses the protein truncation resulting from a nonsense mutation.
Complete Question?
Mutations that result in Amber codon (UAG) suppression in E. coli occur only in tRNA genes that exist in multiple copies. In contrast, Ochre (UAA) and Opal (UGA) suppression sometimes occurs through the use of tRNAs that are encoded by a single, unique gene. Can anybody Explain why Amber suppressors never derive from single-copy, unique tRNA genes, whereas this is sometimes the case with Ochre and Opal suppressors?
Amber suppressor mutation occurs only in tRNA that is in multiple copies, tRNA is essential for viability, if there is only one copy of tRNA and that is also got mutated the cell will die. Therefore amber suppressor mutation always occurs in one of the tRNA so that you have another funtional tRNA for cell to survive. The strain having an amber suppressor muation and is viable will always has one copy of wild type tRNA.
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