Sammuel Shahzad

8 Questions 19 Answers 0 Followers

Questions related from Sammuel Shahzad

Please recommend me a commercially available Expression vector which i can use to add GST tag to my protein?

I want to add a GST tag to my protein. Looking for commercially available expression vector.

15 May 2023 5,064 2 View

What would be binding and washing conditions in Pulldown assay if you are after the unknown partner?

I am trying to find the binding partner of Msp1b1 (outer surface protein of Anaplasma marginale) on DAE 100 (Tick cells). First, I tried Ni-NTA beads and my binding buffer contains 20mM Tris...

05 May 2023 8,796 2 View

How to stain isolated nuclei for RNA seq ?

Please share the protocol

10 March 2023 9,171 2 View

Why is my qPCR Plasmid series not working?

I have inserted a gene block containing primers flanking a random sequence of 300bp into a plasmid. The plasmid was amplified and purified. We have tested it with PCR to confirm the primer...

25 February 2019 8,531 4 View

How to assemble forward and reverse Sanger sequence?

I got forward and reverse sequence of my target amplicon. i want to assemble them i mean AT and CG .Please recommend me some web applet or bioinformatic software

18 January 2019 4,906 8 View

How can i test pcr inhibitors in unknown sample?

I want to know that does my tick DNA contains pcr inhibitors or not?

12 January 2018 6,992 4 View

Which one is best software for multiple sequence alignment and primer designing?

i am using ugene but unable to get the pdf of alignment

27 January 2017 1,787 3 View

In E.coli, mutation in Amber codon (UAG) suppression occur in tRNA genes that exist in multiple copies but in (UAA)& (UGA) 1 gene do this?why is so?

A non sense suppression is a suppression which is due to stop codon i.e,s codon get mutated and resulted in stop codon and truncated protein will produce then a nonsense suppressor will play the...

26 November 2016 7,064 1 View