What is the need of having weights at the output layer of an RNN ?

In RNN, Yt is the output which is given by the equation; Yt = Why.ht where Why is the weight associated with the output layer and ht is the current state. What is the need of having this weight...

11 January 2021 8,710 4 View

Which wave numbers (absorption peak) of a FTIR spectrum indicate the presence of copper, sulfer, NH4 ?

Using FTIR data we want to recognize the presence of Cu, S, NH4 in the fibrous material, jute.

24 December 2020 5,161 3 View

Are the citizens of CIS countries better off now in term of welfare or satisfaction after three decades of change in regime?

I am a research scholar from India. I wish to write a paper on CIS ( ex- communist) countries. I welcome to any researcher with strong idea about CIS countries. It will be joint effort intended...

02 December 2020 9,917 1 View

Why my IP injcetion drug for mice turn opaque after thawing?

Hi, I have prepared a drug solution that is dissolved in DMSO. We are giving IP injection to mice. The final stock concentration is 2mg/ml in 5% DMSO, 2% Tween-80 and 93% Saline. Before injection,...

18 November 2020 6,000 2 View

How to tackle the problem of constant val accuracy in CNN model training ?

I am new to the field of Neural networks. Currently, I am working on training a CNN model to classify XRAY Images into Normal and Viral Pneumonia. I am facing an issue of Constant Val accuracy...

29 October 2020 3,510 10 View

Can a defect level energy be higher than the indirect bandgap of a material?

The emission energy from PL is coming higher than the indirect bandgap of the material? What can be interpreted from this? Is it possible to have a defect level along 'K axis in the band structure...

15 September 2020 8,015 6 View

After what age we can ask questions directly to the children?

Upto which age group of children, we need the permission of parents? For which age group of children, we need to ask the parents, in case we need any information about them?

19 July 2020 7,751 2 View

How do I fix radical anion on a particular molecule of a dimer using Gaussian 09?

I did TD-DFT calculation of a dimer containing radical anion. Here, charge=-1 and multiplicity=2 in the Gaussian input file. After TD-DFT of that dimer containing two different molecules (let's...

11 July 2020 8,322 3 View

What are the methodologies for sedimentary provenance analysis?

In sedimentary provenance analysis, we use the petrographic methods, geochemistry, heavy mineral analysis, etc. I need the methodology for each technique to determine provenance. if possible then...

11 June 2020 8,530 4 View

What are the steps to create velocity field for flood prediction in a basin with ArcGis?

I am working to predict flood in a basin of NE India with ArcGis, but I am not able to make the velocity field for it. Give me some suggestions to do my work.

25 March 2020 7,633 3 View

Why does blocking with my immunogenic peptide cause nuclear fluorescence in ICC?

Hi all, I am validating a polyclonal antibody raised against human ANT4, through WB and ICC. In most of my controls, the antibody shows very little off-target binding, but when I pre-incubate the...

03 March 2021 5,999 3 View

No title

02 March 2021 3,060 3 View

Does Napabucasin soluble in dichloromethane and in ethanol?

I need to prepare a solution of Napabucasin (inhibitor of cancer cell stemness) in dichloromethane. The drug is expensive, so maybe somebody already tried to do it. If it is soluble, what is the...

02 March 2021 9,945 3 View

Help request in CST microwave?

im working now on TPV solar cells and metamaterials and i realy need our help in CST microwave i have a tpv cell simulation and i want to extract particullarly the absorbtion of only the active...

02 March 2021 2,246 2 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View

What could be some reasons why unstimulated monocytes are getting activated?

Im doing PBMC isolation -> CD14+ enrichment using magnetic beads -> stimulation setup. My negative control is just cells in cRPMI but they seem to get activated over and over again.

28 February 2021 7,883 3 View