I've been using Brij-35 as a component for both wash buffer (1x PBS) and diluent buffers (1x PBS-1%BSA) in my inhibition ELISA experiments. I also use 1x PBS-1%BSA for blocking. Now, whenever I substitute Brij-25 with Tween 20 (0.05%) in both wash and diluent buffers, my relative potencies drop significantly with accompanying T-test failures. The same is observed when switching Brij-35 to Tween 20 in just the wash buffer or just the diluent buffers. What could be some plausible explanation for this decrease in relative potency when using Tween 20?
Thank you in advance for everyone's help.
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