In competitive inhibition ELISA, why does Tween 20 decrease potency as opposed to Brij-35?

I've been using Brij-35 as a component for both wash buffer (1x PBS) and diluent buffers (1x PBS-1%BSA) in my inhibition ELISA experiments. I also use 1x PBS-1%BSA for blocking. Now, whenever I...

21 December 2023 9,418 0 View