We are dissociating deep pyramidal cells from 3-4 week old mice. We are using a Worthington Papain Dissociation System. We are wondering if there is another enzyme that would be more beneficial and effective.
I've been using Brij-35 as a component for both wash buffer (1x PBS) and diluent buffers (1x PBS-1%BSA) in my inhibition ELISA experiments. I also use 1x PBS-1%BSA for blocking. Now, whenever I...
20 December 2023 9,442 0 View
I have an issue in analyzing the graph to determine the IC50 for drug A and C which are categorized as a mitotic inhibitor and B is HDACi. I know the possible reason is could be mitotic slipage...
25 September 2016 7,315 7 View
I am currently undertaking my PhD on drug combination against nasopharyngeal carcinoma, NPC. I am screening various targeted therapies and chemotherapies with aim to come out with the best...
21 February 2016 6,299 4 View
Does anyone know of any histories of Catholic social activism between 1890 and 1945 in the United States? Names and autobiographies/biographies of activists would be helpful in addition to social...
09 October 2015 9,532 4 View
My university's -80 freezer just broke down and the repair will take 4 months according to the vendor. Is there any other way to preserve cells? Maintaining lot of flasks until the freezer is...
12 April 2015 5,115 6 View
Hi, I would like to know how important it is to have specific field of study stated in my transcript? My background is in cancer research so I thought that Biotechnology or Applied Biology is not...
01 January 1970 9,690 4 View
Hi, I am having some issue with our rocker. It broke down and it will take time to be fixed. Is there any other solution for me to proceed western blot without rocker? It seems to be impossible....
01 January 1970 1,438 4 View
Hello, I am currently having problems with RNA extraction. I am using mouse liver (C57BL6J), and I have extracted RNA from mouse liver before. Before this experiment, my final RNA pellets were...
11 August 2024 7,082 3 View
Can anyone share any reference that gives mouse specific CD3 antibody sequence. I have been searching for this since few days and no luck. Thank you very much
08 August 2024 9,157 4 View
I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...
07 August 2024 7,535 4 View
Hello everyone, I have recently started using the microtome device for sectioning of paraffin-embedded mice lung. While I had some success in sectioning and observing proper ribbons, some of my...
06 August 2024 666 4 View
Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...
06 August 2024 641 2 View
Hi all, I was just wondering if anyone has experience with multiplexing a mouse monoclonal primary and a rat primary. I'm trying to multiplex by incubating them in the same well but was told by a...
06 August 2024 9,710 1 View
i have sorted anti-NP specific plasma cells from bone marrow of C57BL/6 mice at certain times after immunization with variable counts and isolated total RNA using TRIZOL method for RT-PCR using...
05 August 2024 8,835 1 View
I will be with my students collecting seaweed samples in a marine farm and later we will process this tissue for RNA isolation and further sequencing. Does anyone have tips on how to collect the...
04 August 2024 501 2 View
I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...
04 August 2024 9,913 7 View
Hello, everyone. I have tried to determine carrier motilities of some materials, by Density Functional Theory, using Quantum ESPRESSO. There are a few methods to do it, like a package called...
04 August 2024 8,894 1 View