Can somebody explain about Imprinting and Epigenetics ? How does it play a role in genetic disorders ?
In autosomal dominant disorders I understand that the expression of one allele is required for the proper functioning. In case of mutations why not the protein from the normal allele get...
31 December 2014 4,454 6 View
Usually how many days does it take for mesenchymal stem cells to attain 80-90% confluency?
10 November 2014 2,090 1 View
Can somebody share protocol for culturing mouse iPSC?
10 November 2014 8,970 3 View
Why does tRNA recognize ATG as the first codon?
05 June 2014 2,367 11 View
Why does ddGTP gets incorporated faster than other three ddNTPs during sequencing PCR?
04 May 2014 7,550 0 View
Is there any online tool to analyse frameshift mutation?
04 May 2014 2,203 6 View
Which is better for GC rich amplification?
03 April 2014 1,881 9 View
.
03 April 2014 9,849 5 View
see above
01 February 2014 9,152 9 View
01 February 2014 7,674 5 View
I aim to be as skeptical as possible regarding whether a pair of orthologous genes results in the same phenotype in their different but related bacterial organisms under similar environmental...
05 August 2024 6,787 4 View
I am performing ligation of the plasmid and a target gene. The steps I have taken are: 1. Double digestion of the plasmid and target gene 2. Ligation of the plasmid with the target gene 3....
05 August 2024 2,570 3 View
TEP presentation caption (The Environmental Project) Re: Why should Washington’s DC, or any country government point of location think of as nowadays of as to being 'tomorrow as to come! if it...
03 August 2024 2,484 1 View
On a personal note, even though technology has attracted a lot of interest and funding to combat climate change, it is becoming more and more clear that taking care of the "Human Dimension" is...
02 August 2024 6,773 12 View
I have an RNA-seq data that I have analysed using Limma-voom and have extracted the gene IDs, log2FC and the p-values. At p value < 0.05, I have over 10,000 DEGs, however, when I run the GO...
31 July 2024 225 2 View
I am currently working on a project involving liposomes and need to determine the maximum volume of siRNA that can be added to a 2.5 mL liposome solution with a total lipid concentration of 10...
30 July 2024 6,420 1 View
Hello , I established a stable cell line expressing GFP tagged to a centrosomal gene having G418 drug selection marker. I validated the stable line by IFA and Western blotting, results are fine....
29 July 2024 5,007 0 View
I am writing to request a correction regarding the affiliation of one of my co-authors, Donald Ho. Currently, the author's profile incorrectly lists an affiliation with The Hong Kong Polytechnic...
28 July 2024 6,824 0 View
Since 2016 Brexit, the world needed to change the thinking behind traditional democracy as the democratic landscape changed, yet traditional democratic thinkers and actors have been acting as if...
28 July 2024 6,515 1 View
Perfect democracy thinking assumes no chaos so no need for independent rule of law system and liberal democracies assume the possibility of normal democratic chaos that can be sorted out by an...
28 July 2024 473 1 View