If total RNA extraction contains the Mitochondrial RNA???

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What is the reason film thickness increased?

what is the reason film thickness increased when increased doping concentrations give some reasons The measured film thickness is 156, 282, 394, 548 and 681 nm for 0, 1.5, 2.5, 3.5, and 4.5 wt....

17 February 2021 8,987 5 View

Phytic acid limits bioavailability of micronutrients: Is challenge for biofortification?

As many researcher have confirmed that phytic acid content basically present in cereals and legumes crop that responsible for limiting bioavailability of micronutrients (Fe and Zn) in seeds. Lets...

31 January 2021 5,556 8 View

Requesting dataset for research purpose?

I am doing driving style analysis research with OBD data. Could anyone provide me links to get the dataset?I am not able to get it anywhere..

20 January 2021 9,707 2 View

Is a person who smokes at a higher risk of getting affected by corona virus than a non-smoker?

Is a person who smokes at a higher risk of getting affected by corona virus than that a non-smoker?

14 January 2021 4,246 30 View

BIM Conferences/ Symposium 2021?

Hi, I am Looking for BIM conferences and Symposiums for the year 2021, in order to send my paper. Please do let me know if anyone knows where I can find a list of upcoming Events in BIM. Thank you

30 November 2020 3,978 9 View

Which tool can be used to identify direct (or) inverted repeats in a gene?

I am trying to find out the presence of short repeats (both direct and inverted) in a 3-4 kb long DNA fragment. Although I had tried with a few free online tools, the results were not...

16 October 2020 8,368 3 View

Biofilm Biofertilizers- New Generation biofertilizers?

Biofilm Biofertilizers-It is being applied to adhere the surface of soil and plant matrix containing consortia of various micro-organism that cut down chemical fertilizer. It is cost effective...

12 October 2020 4,883 13 View

Ethics in research publications?

In present scenario, lots of work were done by researchers and no doubt some of these have potential to change mindset of others that visible through benefiting farmers and other stakeholders....

09 October 2020 4,087 7 View

Can the same data be used for both within group correlations and between group mean comparisons?

I have two different measures from two different groups. Both M1 (measure 1) and M2 (measure 2) from both G1 (group 1) and G2 (group 2). Within each group, I want to find correlation between M1...

08 October 2020 1,847 5 View

Effect of the an extreme weather event on milk yield across 100 farms. Do you think the below models reflect the research question?

I have a panel data of monthly milk yield as a dependent variable and extreme weather ( in terms of percentile-based days) and other independent variables on the other side of the equation. The...

04 October 2020 8,960 1 View

How to increase a model accuracy ?

dear community, my model is based feature extraction from non stationary signals using discrete Wavelet Transform and then using statistical features then machine learning classifiers in order to...

03 March 2021 6,994 5 View

Gel electrophoresis result for total RNA samples, 1.5 Agarose gel was used, How to improve the integrity of RNA?

Gel electrophoresis, RNA degradation, RNA extraction from fresh tissue

02 March 2021 5,433 5 View

Problem with shRNA transfection. Why are only two plates getting contaminated instead of all?

I transfected my LNCaP-WT cells with 3 shRNA plus their NTC two weeks ago and split two puromycin selected cell plates on Friday last week(Feb 26). I checked for GFP in the cells, and they all...

28 February 2021 4,949 3 View

Any specific pipeline or parameter to analyse total transcriptomics study of brain tissue samples?

I have two groups of brain samples, control and treated for example. It was total RNA nova seq sequencing. I tried all the available pipeline like: star+rsem+deseq2, Hista+stringtie+cuffdiff,...

27 February 2021 356 6 View

Why is the efficiency of viral mRNA electroporation in PBMC low?

Hello, I have been struggling with the electroporation of PBMC with HIV-I mRNA. Although the quality of the RNA generated is good, and the handling of the cells in meticulous, the frequency of...

27 February 2021 9,233 3 View

Is there any one who can provide a donation of HPV detection and genotyping kits in exchange collaboration fors?

Warm greetings My name is Ayichew S. and I am doing my Ph.D. in Medical Microbiology (on Human papillomavirus). At this stage, I have almost completed a clinical sample Cervical swabs)...

25 February 2021 1,660 3 View

Is there any one who can provide a donation of HPV detection and genotyping kits in exchange collaboration forms?

Warm greetings! My name is Ayichew S. and I am doing my Ph.D. in Medical Microbiology focusing on Human papillomavirus). At this stage, I have almost completed a clinical sample (Cervical swabs)...

25 February 2021 3,612 3 View

What urea buffer can i use for biotagged protein extraction from streptavidin beads?

Hi all, I have been doing research on biotagged LDB1 proteins in Mel cells. I purify the proteins using M280 streptavidin beads. The yield is very low so I've been trying to optimize it by...

24 February 2021 5,749 3 View

What is the best concentration of cDNA to be used for qPCR?

I have synthesised cDNA from 500ng/ul of RNA. The concentration of the cDNA Is about 5000ng/ul. Should I dilute my cDNA or just use it as it is for my qPCR? I usually use 2ul of cDNA for a 20ul...

24 February 2021 8,618 3 View

Recommendations on homogenizers for RNA isolation?

I will be performing RNA isolation with frozen lung biopsy samples and am in the market for a tissue homogenizer. I am wanting a homogenizer that will fit in a 1.5ml tube. I was looking at the...

24 February 2021 3,623 3 View