If my DNA conc. is 300 micrograms/ml, what volume should I use if I want to see bands on an agarose gel after electrophoresis?

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The isolation of genomic DNA from fermented milk samples has resulted in smears, what could be the cause?

Hi all, there have been some challenges with regards to genomic DNA extraction directly from fermented milk samples. Any input with knowledge on troubleshooting would be appreciated. I have been...

07 September 2023 394 2 View

What are the best areas to conducting a research for the postgraduate students who are following degree in MBA E-Governance ?

Generally students are just picking some research areas and conducting a research for their final Thesis. What are the best topics that student can choose in their MBA E-Governance post graduate...

22 October 2021 7,876 10 View

Factor Analysis with items measured on different scales?

In my newly developed tool with items measured on 10 point and 4 point likert scales. How can I do FA. Am I supposed to standardize the scores..? otherwise can I do FA without standardize..?...

20 February 2021 2,362 3 View

Removing an item from a scale to increase the cronbach's alpha?

Hello fellow academics. So i have been struggling with a very low cronbach's alpha on a sub scale i.e alpha of .383 to be precise. After following the advice of some academics on research gate i...

05 July 2020 6,696 1 View

I need some assistance in sourcing a supplier for nylon or Dacron tipped non-wooden swabs for Covid-19 testing?

Hi nylon or Dacron tipped non-wooden swab.

13 May 2020 419 0 View

I need to stain protoplast with Rhodamine B. Pls advice me to prepare Rhodamine for protoplast staining and observation?

How to prepare Rhodamine B for staining protoplast? How to stain? What is the excitation level i should use to observe stained protoplast?

27 November 2017 2,885 3 View

Further management: mCRC with DPG deficiency?

Hi, does anyone have an information regarding further treatment options for a 61 year old male with mCRC with secondaries to the left lung and mediastinal nodes who has DPG deficiency confirmed...

03 September 2017 1,608 1 View

Please help. I have been trying to grow Chlorella vulgaris in 5g/L glucose in BG-11 medium. It grows for 3 days and then dies. Any reasons?

I have tried this about 4 times and it shows the same pattern. In the presence of sucrose, fructose etc., growth continues after 3 days. But in glucose, it just won't grow. All articles indicates...

11 August 2017 2,275 0 View

Have any of you used, or are aware of, pragmatism being used as a theoretical framework for conducting research?

We are aiming to use pragmatism for conducting research in the field of Occuaptional health and safety. I understand this is the preferred theoretical framework for conducting mixed-method...

17 July 2017 6,391 3 View

Anyone aware of research on the impact of mindfulness training on vicarious trauma in mental health care workers?

Seems to be a dearth of academic research on mindfulness interventions in the South African context

23 April 2014 8,133 10 View

Can I base on reverse DNA sequences to perform alignment, convert to amino acids and GenBank submission?

I have reverse sequences (AB1 format), can I base on reverse DNA sequences to perform nucleotide alignment, convert nucleotides to amino acids and deposit the sequence in GenBank database?

11 August 2024 5,138 1 View

I can't see the ssDNA band after performing asymmetric PCR. Is there any way to do this?

After performing symmetric PCR, PCR purification was performed. Afterwards, asymmetric PCR was performed using the PCR purification product as a template, but no ssDNA band was confirmed in the...

08 August 2024 1,668 3 View

Does crude extraction using NaOH and Tris work well with Fungi?

I'm trying to find a DNA extraction method for fungi that does not require equipment and heating. Is there anyone who can suggest an alternative option? Thank you

08 August 2024 4,733 2 View

Why is my ASO degraded quickly on agarose gel?

I have been working on Red blood cell-derived extracellular vesicles as Antisense Oligonucleotide (ASO) carriers. We normally run agarose gel to quantify the loading efficiency. I used naked ASO...

06 August 2024 3,130 2 View

Why after performing site directed mutagenesis ,I don't see any colony after transformation?

I want to introduce a point mutation (change in one nucleotide) into my gene of interest (DNA binding domain) I have designed primers as recommended on the Data sheet of the kit : -Both primers...

05 August 2024 9,059 3 View

Does anyone have issues using Prepman Ultra reagent for MicroSeq ID bacterial, fungal and yeast sample preparation?

I have been attempting to extract DNA from Bacterial, Fungal and Yeast banked samples (>1e7 cells) using Prepman Ultra reagent and I seem to be struggling to obtain a sequence. Although the...

01 August 2024 2,079 0 View

I'm optimizing a tetra-ARMS PCR protocol with amplicon sizes: 165bp, 123bp and 93bp. Why, in the gel, only 165bp is visible while I'm expecting all 3?

It's an end-point PCR protocol. I'm using 1.5% agarose gel with SyBR Safe dye and TBE as a running buffer, visualization on BioRad XR+ system. I was primarily thinking of primer efficiency,...

01 August 2024 4,673 4 View

Inquiry on Maximum Nucleic Acid Volume for 2.5 mL Liposome Solution?

I am currently working on a project involving liposomes and need to determine the maximum volume of siRNA that can be added to a 2.5 mL liposome solution with a total lipid concentration of 10...

30 July 2024 6,420 1 View

Are the apoptotic cells is positive for γH2AX ?

It has been documented that apoptotic cells themselves can induce phosphorylation of serine 139 on H2AX (γH2AX) due to DNA fragmentation during apoptosis (doi: 10.1074/jbc.275.13.9390). As γH2AX...

28 July 2024 7,983 2 View

Can anyone help me explain my problem?

Hi, Now I am doing a single-base editing by using HiFi Cas 9, guideRNA, single stranded DNA, HDR Enhancer V2 and my tranfection mehtod is lipofection (RNAiMax). Unfortunately, after gene editing,...

26 July 2024 2,136 0 View