Removing an item from a scale to increase the cronbach's alpha?

Hello fellow academics. So i have been struggling with a very low cronbach's alpha on a sub scale i.e alpha of .383 to be precise. After following the advice of some academics on research gate i...

05 July 2020 6,536 1 View

Have any of you used, or are aware of, pragmatism being used as a theoretical framework for conducting research?

We are aiming to use pragmatism for conducting research in the field of Occuaptional health and safety. I understand this is the preferred theoretical framework for conducting mixed-method...

17 July 2017 6,263 3 View

What is the minimum concentration of DNA needed, in order for bands to be observed on a 1% agarose gel after electrophoresis?

I need to run an agarose gel that has restricted DNA however I need to know the minimum amount of dna that can be seen on the agarose gel. I presently do not get visible clear bands due to the...

15 September 2013 7,005 5 View

If my DNA conc. is 300 micrograms/ml, what volume should I use if I want to see bands on an agarose gel after electrophoresis?

Only 2 microL of enzyme and buffer can be used.

15 September 2013 6,991 2 View

Buffer to disrupt platelet alpha granules for PF4 quantification?

Hello! I want to quantify by ELISA the secreted (from platelets poor plasma) and the non-secreted (from platelet lysate) PF4 before and after TRAP stimulation. I will use the ELISA from R&D...

03 March 2021 1,499 2 View

Dapi fluorescence protocol for colon cell lines ?

28 February 2021 2,534 2 View

Can I use acetic acid with an ELITE WAX Perkin Elmer column for Gas Chromatography?

Hi! I would want to know if I can use acetic acid with an ELITE WAX Perkin Elmer column for Gas Chromatography. This column has a polyethylene glycol phase, but I can't find the manual to check...

22 February 2021 730 2 View

Polymer chain length? Coiled length and the extended length of an average molecule?

A sample of Polyethylene has an average degree of polymerisation of 1200. What is the coiled length and the extended length of an average molecule given the bond length is 0.154nm and the bond...

10 January 2021 6,942 3 View

Why Selenium nanoparticles synthesized by PEG 200 could not be scaled up ?

I could not synthesize Selenium nanoparticles by PEG 200 at up-scale ( more than 100 ml) as it got aggregated while at small amounts it works fine , I work with 850 PPM using selenium powder in...

08 January 2021 10,049 1 View

How can I fix the density of system in coarsed grain simulation?

In the process of obtaining the nonbond potential of the Coarse-grained(CG) model, I need to simulate the CG model mapped from the conformation of all atom(AA). AA simulation process is...

02 January 2021 3,643 1 View

Does anyone have suggestion on how to sterilise high Mw polyethylene oxide (PEO) to be used for cell cultures?

I am trying to sterilise high molecular weight polyethylene oxide (~400,000 Da) in DMEM for culturing bmMSCs. I have done online search for relevant information, but most studies were using PEG...

25 November 2020 3,134 8 View

How do I make nano-sized particles of Polyethylene?

I am attempting to make particles in the range of 50-500 nm from Low density polyethylene. My current protocol is to dissolve 0.1 g of LDPE in xylene at 100 C, then drip the resultant solution...

08 November 2020 7,764 1 View

Looking for non-toxic super absorbent and hydrogels with low flexural strength?

I am looking for a non-toxic and ineffective hydrogel that can be converted into fibers by electrospinning. I also need to know its solvent. Since I am going to use these fibers for medical...

14 October 2020 9,936 8 View

What are the main problems associated with LDPE recycling or valorization compared to other polymers such as HDPE and PET?

In most developing countries like Nigeria, low to medium-income earners consume packaged water coming from sachet water. Sachet water is made from LDPE. I am looking at each packaging holistically...

13 October 2020 5,044 3 View