Facing a problem to circularize the bacterial plasmid during hybrid assembly in unicycler. What's the solution?

30 July 2023 1 1K Report

I am facing a problem to circularize a particular bacterial plasmid sequence while doing hybrid assembly in unicycler. After the assembly, it's not showing circular. While the other plasmids have circularized in unicycler after doing the hybrid assembly. Please give me a solution how that particular plasmid can be circularized during hybrid assembly?

Abhijeet Singh

If you are not getting that particular plasmid circularized while others were circularized, you should get an idea what could be the problem. Either there are some parts missing or there might be the quality issues. Thus, these issues are probably not easy to fix based on the data. If you are changinf the paramters, you might accomplish your task but there might be a trade off on the quality.

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