How can I know the product size in base pair, while I don't have positive control and the reference paper did not mention the expected product size?
use the BLAST tool at NCBI and BLAST each primer sequenc against the genome you are working on, It will give you a position on the genome and subtracting the 2 positions from each other will give you the size between the primers
If the reference paper did not mention the expected product size, and you do not have a positive control, you can try to estimate the size of the PCR product based on the sequence information of the primer pair.
You can first perform a BLAST search to identify the primer sequences in a reference database, such as NCBI's nucleotide database. Once you have identified the sequence of the PCR product generated by the primer pair, you can use a tool such as Primer-BLAST to design new primers targeting the region of interest with a known expected product size.
Another option is to estimate the size of the PCR product based on the length of the primer sequences and their distance from each other on the reference genome or sequence. This can give a rough estimate of the expected size of the PCR product.
The actual size of the PCR product may vary depending on the specific conditions of your experiment, such as the annealing temperature and cycle parameters used in the PCR reaction. Therefore, it is always recommended to run a positive control to confirm the expected size of the PCR product.
These video playlists might be helpful to you:
https://www.youtube.com/playlist?list=PLzBiAPKi8vONSqjcweFWHMRy13y3484bE
https://www.youtube.com/playlist?list=PLzBiAPKi8vOMheKwb0mu1dcZRaeGUFSlw
https://www.youtube.com/playlist?list=PLzBiAPKi8vOPbE7Db9mKwloe34E8rXXEU
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