Can the CSF-1R antagonist PLX3397 be administered via minipump to deplete microglia?

Hi All, Interested in depleting microglia using PLX3397. I have read dozen of studies that formulate this into rat chow - or use oral gavage. I would preferably deliver the drug chronically...

10 November 2018 3,015 2 View

Best affordable off the shelf strategies to limit angiogensis in the mouse?

We have an approach that we are evaluating in a mouse model of stroke which stimulates vessel development. We would like to show that vessel development is the key mechanism responsible for the...

07 August 2018 4,052 4 View

Does anybody have images of what a microglial macropore looks like?

They are frequently referred to but not commonly imaged.

06 July 2016 4,842 4 View

DMSO in frozen whole bloods: What is the best way to get rid of it?

We have some human frozen whole bloods available to us (+DMSO). I would like to thaw them and run a whole blood cytokine stimulation assay +/- LPS +/- dexamethasone. How should I get rid of the...

08 September 2015 9,454 5 View

To what extent are psychometrics used to evaluate chronic stress, such as the PSS, influenced by recency effects?

10 November 2014 8,137 9 View

Which one is correct: Transgenic GFAP-EGFP labelling or GFAP immunolabelling?

Today, we processed tissue from our first GFAP-EGFP mouse (on a C57B6 background). Tissue was fixed with 4% PFA and imaged using a 1.4NA 63x oil immersion lens. We compared this side by side with...

05 June 2014 840 4 View

Does anybody use an automated machine for research related IHC?

If so could you make a recommendation? We do mountains of IHC in the lab and I would love a machine to improve efficiency and throughput.

01 February 2014 3,080 3 View

What is the secret to good immunofluorescent S100B labelling?

We have got very good DAB labelling using Sigma monoclonal directed towards S100B in the rat but our immunofluorescence is rubbish. We've run to serial dilutions using the antibody ranging from...

08 September 2013 259 1 View

What are the optimal/sensible breeding strategy for animals carrying transgenes?

I would very much like to start using transgenic mice that posses promoters that drive the expression of flurophores for in-vivo imaging. What I didn't fully appreciate at the time that I ordered...

06 July 2013 5,337 14 View

Are transgenic mice expressing a red flurophore under the control of a microglial promoter available?

At present I am not aware of the availability of any transgenic mice expressing a red flurophore under the control of a microglial specific promoter. Currently, mice available from Jackson (CD11b...

06 July 2013 6,740 12 View

How to define and get results (stresses) for certain points of a model in abaqus, using python? When the size of the model changes?

I want to do a parameter study of a aixsymmetric cylinder in Abaqus. I want to create several jobs where I get the stresses for 3 different points in my model. These points are defined. I wrote a...

01 March 2021 10,078 1 View

LOAD DISPLACEMENT CURVES Stiffness increasing in ANSYS non linear analysis. HOW CAN I CORRECT IT?

Hi. I am doing non linear FE analysis using a new material beam under flexural 2 point loading using ANSYS. I have defined a bilinear material model. The solution has converged. In results I am...

01 March 2021 9,911 3 View

How to simultaneously perform multiple biochemical assays?

I am a research scholar working on heavy metal stress on plants. I will be doing biochemical characterisation( protein, carbohydrate, proline, antioxidant enzymes and many more assays) at interval...

01 March 2021 6,999 3 View

Tooth extraction and subsequent dental implant placement in Sprague-Dawley rats induce differential changes in anterior digastric myofibre size and ?

01 March 2021 7,254 3 View

How relevant is Mini slump cone?

for all the rheology researchers, my question is, how relevant is a mini slump cone (smaller than a standard 50 cent rheometer). currently based on testing, the thickness of slumped material is...

26 February 2021 3,668 1 View

Stress/strain tensors decomposition ???

25 February 2021 6,760 1 View

How to reduce residual stresses in MD simulation?

I am working on a preparation of representative polymeric structures of some popular polymers, such as polyimide or PTFE for MD simulation, during which a single nitrogen or oxygen atom will be...

24 February 2021 8,105 3 View

Suggestions for occupational stress surveys?

I am currently writing a masters thesis on reasons for occupational stress in the early childhood classroom, and want to include a short survey assessing participants work-related stress levels. I...

24 February 2021 8,678 2 View

The most suitable method to calculate the stress intensity factor in the presence of contact between the crack lips?

I'm trying to study cracked structures under a compressive load taking into account contact between the crack lips. I would like to know if the method of extrapolation by displacement remains...

21 February 2021 8,694 4 View

How to solve 2nd order non-linear and non-homogenous ordinary differential equation using non-linear shooting method and RK4 using Python?

I want to solve a second-order non-linear and non-homogenous order differential equation using the non-linear shooting method and fourth-order Runk Gutta using Python? Thank you for your help!

21 February 2021 3,225 3 View