18 Questions 78 Answers 0 Followers
Questions related from Frederick Rohan Walker
Hi All, Interested in depleting microglia using PLX3397. I have read dozen of studies that formulate this into rat chow - or use oral gavage. I would preferably deliver the drug chronically...
11 November 2018 3,106 2 View
We have an approach that we are evaluating in a mouse model of stroke which stimulates vessel development. We would like to show that vessel development is the key mechanism responsible for the...
08 August 2018 4,147 4 View
I would like to start examining how stressors alter go-no go performance. Specifically, I would like to get a program - that could run in VR that would allow me to alter the frequency of go - no...
07 July 2017 5,177 0 View
They are frequently referred to but not commonly imaged.
07 July 2016 4,953 4 View
We have some human frozen whole bloods available to us (+DMSO). I would like to thaw them and run a whole blood cytokine stimulation assay +/- LPS +/- dexamethasone. How should I get rid of the...
09 September 2015 9,532 5 View
Also there is quite a bit of research to indicate that accuracy of autobiographical recall deteriorates quite rapidly. Are these issues widely recognized as important in interpretation of data...
11 November 2014 8,220 9 View
Today, we processed tissue from our first GFAP-EGFP mouse (on a C57B6 background). Tissue was fixed with 4% PFA and imaged using a 1.4NA 63x oil immersion lens. We compared this side by side with...
06 June 2014 930 4 View
If so could you make a recommendation? We do mountains of IHC in the lab and I would love a machine to improve efficiency and throughput.
02 February 2014 3,156 3 View
We have got very good DAB labelling using Sigma monoclonal directed towards S100B in the rat but our immunofluorescence is rubbish. We've run to serial dilutions using the antibody ranging from...
09 September 2013 354 1 View
I would very much like to start using transgenic mice that posses promoters that drive the expression of flurophores for in-vivo imaging. What I didn't fully appreciate at the time that I ordered...
07 July 2013 5,420 14 View
From experiments in which Astrcoytes have been filled with fluorescent dyes, such as Lucifer Yellow, it is clear that GFAP only identifies a modest proportion (maybe 15%) of the cells total...
07 July 2013 8,107 25 View
At present I am not aware of the availability of any transgenic mice expressing a red flurophore under the control of a microglial specific promoter. Currently, mice available from Jackson (CD11b...
07 July 2013 6,827 12 View
Lavision biotek and Zeiss now have commercially available light sheet systems. The approach looks interesting because when combined with clearing agents, and fluorescently labelled structures it...
04 April 2013 5,307 5 View
We have had some problems with z-stacks that are slightly even due to sectioning artefacts - i.e one side of a section being slightly higher than the other. This creates some problems when...
02 February 2013 9,768 14 View
We are interested in constructing an intrinsic optical imaging system to investigate changes in cortical blood flow. The simple versions of this procedure - look relatively straight forward to...
02 February 2013 1,381 19 View
In the last 12 months we have started to look at imaging CNS tissues using TEM. The ultimate goal for us is to do pre-embedding IHC. Very quickly we identified that conditions that suited IHC...
12 December 2012 3,074 7 View
It is my understanding that the best, and most well characterised drug for inhibiting microglial activity is minocycline. It is clear, however, the drug can influence some neuronal functions....
09 September 2012 1,332 21 View
How straight foward is it to create a large image montage using images acquired using a microscope using a motorized XYZ stage?
08 August 2012 621 7 View
