Can the CSF-1R antagonist PLX3397 be administered via minipump to deplete microglia?

Hi All, Interested in depleting microglia using PLX3397. I have read dozen of studies that formulate this into rat chow - or use oral gavage. I would preferably deliver the drug chronically...

10 November 2018 3,015 2 View

Best affordable off the shelf strategies to limit angiogensis in the mouse?

We have an approach that we are evaluating in a mouse model of stroke which stimulates vessel development. We would like to show that vessel development is the key mechanism responsible for the...

07 August 2018 4,052 4 View

I would like to know whether there is an accessible (free?) go / no-go task that I can run in a vitual reality environment (HTC vive / oculus / Gear)?

06 July 2017 5,087 0 View

DMSO in frozen whole bloods: What is the best way to get rid of it?

We have some human frozen whole bloods available to us (+DMSO). I would like to thaw them and run a whole blood cytokine stimulation assay +/- LPS +/- dexamethasone. How should I get rid of the...

08 September 2015 9,454 5 View

To what extent are psychometrics used to evaluate chronic stress, such as the PSS, influenced by recency effects?

10 November 2014 8,137 9 View

Which one is correct: Transgenic GFAP-EGFP labelling or GFAP immunolabelling?

Today, we processed tissue from our first GFAP-EGFP mouse (on a C57B6 background). Tissue was fixed with 4% PFA and imaged using a 1.4NA 63x oil immersion lens. We compared this side by side with...

05 June 2014 840 4 View

Does anybody use an automated machine for research related IHC?

If so could you make a recommendation? We do mountains of IHC in the lab and I would love a machine to improve efficiency and throughput.

01 February 2014 3,080 3 View

What is the secret to good immunofluorescent S100B labelling?

We have got very good DAB labelling using Sigma monoclonal directed towards S100B in the rat but our immunofluorescence is rubbish. We've run to serial dilutions using the antibody ranging from...

08 September 2013 259 1 View

What are the optimal/sensible breeding strategy for animals carrying transgenes?

I would very much like to start using transgenic mice that posses promoters that drive the expression of flurophores for in-vivo imaging. What I didn't fully appreciate at the time that I ordered...

06 July 2013 5,337 14 View

Are transgenic mice expressing a red flurophore under the control of a microglial promoter available?

At present I am not aware of the availability of any transgenic mice expressing a red flurophore under the control of a microglial specific promoter. Currently, mice available from Jackson (CD11b...

06 July 2013 6,740 12 View

Is there a way in which microglia can be extracted from mice without killing them ?

I'm aiming to use an adoptive cell transfer approach, with microglia in mice with ALS. So far all the protocols I have seen, require sacrificing the mouse. I'd like to re-infuse the microglia back...

11 February 2021 9,509 1 View

Does anyone have a stack of images of action potentials or abnormal brain scans?

I need a stack of black and white .jpeg/.tiff/.jpg/.png images across time of either action potentials, neurons firing, or brain scans (comparing disease and normal brain, disease progression,...

09 February 2021 2,648 3 View

Help needed: How to map AA, AG, and GG genotypes on to polymorphism information?

Dear Colleagues, I am new to genes and genotyping and I need help. I am trying to figure our how AA, AG, and GG genotypes map on to A1 for DRD2/ANKK1 Taq1A polymorphism(rs1800497) and Val or MET...

02 February 2021 3,861 3 View

How can I analyze the morphology of microglia (ramification, braches, soma, etc.) via Fiji/ImageJ?

Hey everybody, for my project I need to analyze the morphology of microglia, which I have previously stained (IHC) and scanned in a SlideScanner. Specifically, I need the ramification, number and...

31 January 2021 3,855 3 View

What are some recent publications showing the predictor power of resting state brain acitivity for tasks done outside the MRI scanner?

Looking for recent articles (2018-2020) that showed resting state brain activity can predict cognitive task performance (e.g., attentional and executive functioning tasks) done in the lab AFTER...

09 January 2021 2,310 2 View

No title

Hi, I am interested to see the effect of BCG on macrophages (microglia )Therefore , I would like to know the amounts and the routes of administration which is safe in mice and how long is...

20 December 2020 7,166 2 View

Did anyone do microglia Oil Red O staining?

I need to stain adiposome in microglia, but I don't know how to confirm that are really success? method : Removed media and washed cells with PBS, then added 4 % paraformaldehyde for 20 minutes at...

24 November 2020 2,359 2 View

Why is the impedance of ECoG electrodes tested at a frequency range from 1 Hz -100 kHz?

I am testing the impedance of a material that will be used for ECoG electrodes. I was told to test it at a frequency range from 1 Hz - 100 kHz but why is this range used? I wasnt able to find a...

08 November 2020 8,089 3 View

Does anyone have experience giving a CSF-1 R inhibitor via IP injection to deplete microglia?

Dear colleagues, I am studying microglia in early development. I would like to design a study where I deplete microglia in neonatal pups using a CSF-1R inhibitor. Several studies that employ the...

06 November 2020 793 2 View

Where can I find coordinates of specific regions of interest (ROI) in the human brain?

Hi All, I need to specify a few Regions of Interest (ROI) in the human brain providing their coordinates (x,y,z). What I am interested in at this point are anterior cingulate cortex, dorsolateral...

01 November 2020 8,442 7 View