I want to separate a lipidic small molecule based on molecular weight, from its complex. Can somebody suggest a possible way in which it can be done?

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Tejaswini Kalkundri

Hi Rashmi,

You can try fractional separation with no-polar solvents such as pet. ether and further purify using semiprep HPLC.

Rashmi Bhosale

Thanks Tejaswini Kalkundri. But, I do not have much amount of the lipidic metabolite to run on a column. It is hardly a few mg.

You can buy small molecular sieves incorporated in Falcon tubes from sartorius. They provide a range based on you're requirement.