Rashmi Bhosale

3 Questions 4 Answers 0 Followers

Questions related from Rashmi Bhosale

How to purify metalloproteins in apo form?

I need to purify a metalloprotein in its apo form. During heterologous expression of protein, there are chances that the protein binds to metal ions from E coli. How can I get recombinant protein...

15 May 2023 9,480 2 View

I want to separate a lipidic small molecule based on molecular weight, from its complex. Can somebody suggest a possible way in which it can be done?

The presence of C12 acyl chain makes it difficult to separate it through the conventional molecular sieve chromatography!

23 June 2022 9,512 3 View

How will higher concentration of beta-Mercaptoethanol affect mammalian cells in RPMI 1640 culture conditions?

I have used 5 ul of 5 times diluted beta-Mercaptoethanol in 200 ml of RPMI 1640 (i.e ~71.4 uM conc) for culturing mouse skin cells.

23 February 2020 1,903 0 View