Fish blood the cells are visible but not micronucleus in the toxicant group.
Dear Vidya Balakrishnan P V,
The protocol followed was as follows :
Smear of blood on clean slide
Fix in methanol for 5 minutes.
Stan in May Grunnwalds (Sol1 for 3 min and sol 2 for 5min)
5% Giemsa for 30 min
Mount in DPX
Can u see micronuclei or any nuclear anomalies in the control group?
Attached one paper. pls go through.
Usually its very easy to perform in vivo mn assay. There will be a minimal number of anomalies even in the untreated controls. so there may be some problem with the protocol or manual error. We should be keen enough to see the abnormalities. First go thoroughly through the literatures and be familiar with the terms, images etc. I think by trial and error and by practice you could visualize, sort out and point out nuclear anomalies.
Good luck
Dr. Vidya
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