How can I measure MMP-1 protein activity or protein level from liver tissue?

Instead of checking only the mRNA level, I want to check the active protein level of MMP-1 in Liver tissue from mice. How can I do that?

03 March 2021 1,763 2 View

Binary Logistic Generalized Linear Mixed model in SPSS. Is it doable?

I want to analyses the proportion of swimming sperm of three species of fish in two salinities. To analyse the proportion of swimming sperm in a Generalized Linear Model, I would use a Binary...

03 March 2021 2,297 3 View

Looking for Materials with high thermal expansion coefficient and high temperature resistance?

03 March 2021 8,272 1 View

Guidelines for survey (question type) analysis?

Hi. Please tell me what guidelines should i need to follow for questionaries' type research work in India. It is not hospital based work, we are conduction basic institutional based qualitative...

03 March 2021 2,037 3 View

What are the values needed for gabor filter's parameter for cifar10 data?

Hi, I implemented a code to gabor filter cifar10 data but the images after being filtered and stacked are not clear like the original images. I think the problem is in the way I am using the...

03 March 2021 6,317 1 View

Can we block unwanted images during the x-ray prediction?

i am try to classify the x-ray images. During classification , can i block unwanted images (except x-ray image).

03 March 2021 7,100 1 View

No title

03 March 2021 5,360 2 View

What techniques can I use to determine the miscibility of polymer blends?

The term miscibility refers to the single-phase state in thermodynamics. I do not mean the compatibility of different components. To determine the miscibility I know several techniques such as...

03 March 2021 4,107 4 View

Sample dilution for quantitative analysis using ELISA?

If the detection range is in ng/ml but the reference range is in ug/ml for a molecule or protein in serum or plasma .how to dilute and what is the initial volume to be taken for quantitative analysis

02 March 2021 7,670 3 View

The procedure compute?

02 March 2021 5,204 3 View

How to calculate Total Flavonoid?

I analyzed flavonoid total spectrophotometry, the procedure as below: 2 ml supernatan added 5 ml dd.H2O, then added 0,15 ml NaNO2 5%, 5 minutes later added 0,15 ml AlCl3 10%, After 6 minutes,...

01 March 2021 6,722 1 View

Phenol-pH graphic?

We want to use phenol pH graphic to measure the pH of mediums before and after inhibitor application to cells. We have phenol absorbence values at 460, 560 and 720 nm wavelength. When we measure...

20 February 2021 7,700 3 View

Can any suggest me good method of GC MS for the separation of Flavonoids?

I prepared the ethyl acetate, ethanol and butanol fraction from the ethanol extract of seed for isolation of Flavonoids. Amongst them ethanol fraction showing shinoda test positive for polar...

13 February 2021 8,263 3 View

Why is there cloudy white solution in flavonoid test tube?

I do flavonoid test for biscuit incorporated with muntingia fruit powder. Sample extracted with metanol (1:10 ,w/v), macerated 24h, sentrifuged and filtered. Supernatan used for analysis. I have...

08 February 2021 6,779 4 View

What is suitable temperature at reaction mixture of lipase activity assay?

I use para nitrophenyl palmitate substrate with tris buffer ph 8... i read many temperature for Yellow color of para nitro phenol production.. 37 and 50 and 40 at water bath ... I confused as i...

04 February 2021 9,100 5 View

Can you use MTS assay with cells cultured in media containing phenol red?

I am trying to run a cell viability assay with LN229, but these cells are cultured in DMEM which contain phenol red. Is the MTS assay sensitive to the additional of phenol red in the media? If so,...

03 February 2021 9,478 2 View

Extraction of phytochemicals from juice?

I am trying to extract phytochemicals from cane juice. I have tried lyophilization and SALLE techniques but the final product is too sticky and sugars are hindering any further extraction. I have...

03 February 2021 8,784 3 View

How to screen phytochemicals directly from the crude plant latex?

Number of methods are available to screen phytochemicals through extract prepared in different solvents including aqueous and organic. Is there any method/ published work related to phytochemical...

01 February 2021 7,348 4 View

I have started a gaussian calculations for DFT analysis of phytochemical, it is showing a error 2070?

I am doing optimization of a phytochemical but when i submitted the file for calculations it shows error 2070. Can anyone please tell me what does it mean? and what should i do to solve it. I have...

30 January 2021 7,932 1 View

How to Extract DNA from dried rhizomes, wood, galls etc. stored for a long time?

Dear Respectable Scientists, I have been trying to extract DNA from above mentioned sources using different CTAB/SDS Buffer Compositions and purifying agents but I am not able to extract DNA from...

12 January 2021 5,899 8 View