I want to seed 10000 cells per well in 96 well plate. generally we add 100ul cell suspension. If we add 200ul cell suspension will it become more than 10000 cells
When explaining substitution models, the substitutions are expressed as Q matrix. Why is the sum of the elements in a row zero?
28 February 2021 3,864 3 View
I am looking forward to remove excess of salt form my metal based porphyrin and trying DSC-18 columns for the same, can someone suggest me how how can one decide the polarity of the solvent to...
01 February 2021 9,076 1 View
I have generated two antibodies against two peptide fractions of a protein in the rabbit. I want to use these two antibodies (Ab-1 and Ab-2) in indirect sandwich ELISA for the detection of...
11 January 2021 1,754 4 View
I have categorized the disturbance basically on movement of people and vehicle in into 3 categories. Disturbed, close to highway and movement of people from dusk to dawn. Intermediately...
30 December 2020 8,806 4 View
I have checked annealing temperature through gradient PCR. I have checked my primer in NCBI. I have checked PCR reagents concentration. Please suggest me anyone thanks
12 December 2020 1,969 2 View
If I am drilling through bars of different material(ex: iron, steel etc.) of the same thickness and under the same conditions of drill operations(feed rate and rpm), how will the torque and thrust...
06 November 2020 4,498 3 View
I have generated an antibody against an antigen. Now, I want to use the developed antibody in the detection of the unknown sample. So, I want to coat the plasma sample as antigen. How that can be...
26 October 2020 2,259 7 View
As we know that the global temperatures are increasing due to climate change which could affect the thermal needs of the crops. I want to make geospatial maps and analysis for heat requirements of...
28 September 2020 8,286 2 View
I am simulating a NW based MSM Photodetector with Si/ CdTe/InAs NWs (modeled in SDE)with moderate doping, and have assumed a constant generation rate in the the Physics Section of SDEVICE 1....
03 September 2020 5,971 10 View
I tried to submit research projects on climate change and Remote sensing to many agencies but could not get it though? Is there any easily available agencies who support the research in the subject.
16 August 2020 4,000 18 View
I am using a 2707 waters HPLC device. When I try to inject a sample, it says missing plate or rack. I changed the needle and calibrated its position but I still get the same problem. I even get...
02 March 2021 1,408 1 View
We have HUVEC bought from Invitrogen. Cat no -C-003-5c. while first plating the cells we have bought the protocol suggests not to centrifuge the cell with media to remove the DMSO. Should we...
02 March 2021 3,713 2 View
Good afternoon, I recently used OmniLog from BIOLOG for my experimentations : I tested the metabolism of different strains on 2 types of plates. I have 16 strains of 3 different groups...
02 March 2021 3,584 1 View
We have always used the 96 welled plate, the 48 welled plate but apparently there is much evidence on the 24 welled plate and above all its benefits.It states it is the same you would use on ELISA...
28 February 2021 1,149 1 View
Hi, recently I encountered a protocol for plating which was not the usual method I have been practicing (pour LB agar onto plate, allow it to solidify, pipette bacterial suspension onto plate,...
28 February 2021 7,292 3 View
Hello, I am looking for some expertise on culturing MCF7s. I purchased them from ATCC and I have been following all media and culture conditions. Media is EMEM with 10% FBS and 10ug/mL of...
27 February 2021 3,904 2 View
Dear all, I am trying to perform a MD simulation about the interaction between CO2 and a polymer. I create my model, solvate it and perform the NVT and NPT equilibration. Now I am not able to...
26 February 2021 10,014 3 View
Hello, recently I am having some difficulties with maintaining the cell culture of primary cells isolated from a skin biopsy (normal and malignant). The coating of culture plates with Matrigel (a...
26 February 2021 6,151 2 View
I am facing difficulties in cloning a 1kb gene into a vector (pJIT163). I have my gene on interest (GOI) in pUC57 and want to clone in pJIT163 using SalI and BamHI restriction sites. I am getting...
25 February 2021 3,221 7 View
Dear peer scientists, Ultradry storage is not suitable for recalcitrant seeds. However, theoretically, if we can stabilize the structure of the subcellular membrane, as well as glassify the...
25 February 2021 2,442 1 View