I want to seed 10000 cells per well in 96 well plate. generally we add 100ul cell suspension. If we add 200ul cell suspension will it become more than 10000 cells
How to determine tannin content in fodder pellets?
22 July 2024 8,163 3 View
Want to authenticate aquatic plant species Duckweed? Two University botanist are unable to confirm, any confirmed place where it can be done in India?
06 June 2024 5,845 0 View
Is it possible to quantify the flow without adding any polymeric beads? I'm interested to know whether there's dominant capillary or Marangoni flow. Aby suggestions would really be helpful.
05 May 2024 2,233 5 View
Today I found a very strange thing in my research gate account..it showing Citations -0..However, I saw more than 150 Citations last week..Dose anyone know how to fix it ??
19 April 2024 1,111 2 View
I want to quantify low cAMP levels generated of a constitutively active GPCR also by different mutants of this receptor. I have tried the cAMP ELISA kit from Cayman chemicals with very...
14 April 2024 7,319 0 View
AI is coming fast, and we have to adapt.
29 March 2024 2,315 1 View
What this minimum of maximum strain of Human cortical Bone before fracture. I saw lot of papers showing different values.
27 March 2024 4,255 1 View
I want to run STRUCTURE on a nuclear gene of an insect species. The sequences that I have received are showing the presence of degenerate nucleotides in the consensus sequences. Do I need to edit...
29 February 2024 648 1 View
In composite material, two elements of the base material show a positive shift in the binding energy, while the third element shows a negative shift in the binding energy. What are the reasons for...
11 February 2024 367 2 View
Hi i am doing work on a protein which has Cu ion in it but the bond it is making with protein are not matching the reference protein making bond with Cu ion so is there any possible to change the...
23 January 2024 5,999 1 View
I have virus (viral hemorrhagic septicemia virus) in suspension and the experiment will not involve cells. What level of TCID50 is preferred?
11 August 2024 3,115 1 View
I am experimenting with cancer and non-cancer cells in a 12-well plate for 4 days with a seeding density 1*10^4/well, however, I noticed that the control group growth rate slows down on D3. Should...
07 August 2024 2,283 2 View
Hi, I have a question about normalizing the MTT OD values for doing the statistical analysis. So, if we have 3 different plates and we call them 3 different replicates, so, first we would...
07 August 2024 8,106 4 View
I have been performing short phagocytosis experiments using 96-round bottom, tissue culture treated, well plates (Falcon, 353077). I culture the cells with the phagocytic cargo for 2-4hrs, wash...
04 August 2024 5,228 4 View
I'm working on camelina seed sterilization and have some doubts about the process. I followed a protocol used by my lab mate, but I'm unsure about a couple of things: Is it safe to use 10% bleach...
02 August 2024 1,178 3 View
In my study, I intend to infect PBMCs with SARS-CoV-2. After that, I will analyze NK cells by flow cytometry to see if their phenotype changes or if they show degranulation. After the infection, I...
01 August 2024 4,403 4 View
Dear researchers, Today, we found this object in one of the wells of a 96wp culture plate. The plate contained MEC-1 cells and GelTrex. I am quite curious what this is (because it's not cells). Do...
01 August 2024 2,250 2 View
Does anybody know of entomological Journals without page charges that would publish an extensive checklist (Hong Kong Aculeates wasps) covering 350 species (of which more than half are new to Hong...
01 August 2024 6,134 2 View
I am trying to adapt adherent CHO-K1 cells in F12K+10%FBS to suspension culture in only F12K, but in vain. I have followed Mirus bio as well as Thermo protocols, please suggest any alternative....
31 July 2024 3,756 0 View
I have been following Rivero et al 2014 protocols, but I am not sure when I should stop watering because my plants are completely yellow but the siliques are not 80% brown. Any recommendations?...
30 July 2024 2,301 7 View