I want to run protein complex in a Native PAGE to achieve the oligomeric state, can anyone help me?

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Sven Falke

Blue native PAGE should be perfect. In BN-PAGE the charge of your proteins will be roughly equally negative. I think a gradient gel with up to around 15% acryl amide should give you good resolution for different oligomers.

There is a good JOVE video on how to analyse more complex samples (doi: 10.3791/2164) by BN-PAGE. There are also different company manuals and ready-to-use components that should help, e.g. here is also a solid protocol by SERVA included: https://www.serva.de/www_root/documents/Blue%20Clear%20Native%20Starter%20Kit%20Ver%20%200215_e.pdf

Good success.

Adeyemi Oluwadare Ayodeji

Blue-native Polyacrylamide gel electrophoresis (BN-PAGE) is a very good method as suggested. Here is an article that explains it as simple as possible.

Goodluck.

Article Blue-native PAGE in plants: A tool in analysis of protein-pr...