I have two different proteins of pI 4.74 with 14.4 kDa size & 8.24 with 45.8 kDa. I would like to run Native-PAGE these proteins separately with some ligands to achieve the oligomeric state.
Could anyone please help me and provide me basic protocol?
I have already followed the basic protocol mentioned below but unable to see and band.
Ready Gel Tris-HCI Gel Composition
Gel buffer 0.375 M Tris-HCI. pH 8.8
Cross-linker 2.6% C
Stacking gel 4% T, 2.6% C
Storage buffer 0.375 M Tris-HCI, pH 8.8
Running Buffer Working Concentration
25 mM Tris - Base
192 mM glycine
could anyone please suggest me the working protocol if used by any?