I have two different proteins of pI 4.74 with 14.4 kDa size & 8.24 with 45.8 kDa. I would like to run Native-PAGE these proteins separately with some ligands to achieve the oligomeric state.

Could anyone please help me and provide me basic protocol?

I have already followed the basic protocol mentioned below but unable to see and band.

Ready Gel Tris-HCI Gel Composition

Gel buffer 0.375 M Tris-HCI. pH 8.8

Cross-linker 2.6% C

Stacking gel 4% T, 2.6% C

Storage buffer 0.375 M Tris-HCI, pH 8.8

Running Buffer Working Concentration

25 mM Tris - Base

192 mM glycine

could anyone please suggest me the working protocol if used by any?

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