How can I measure MMP-1 protein activity or protein level from liver tissue?

Instead of checking only the mRNA level, I want to check the active protein level of MMP-1 in Liver tissue from mice. How can I do that?

03 March 2021 1,763 2 View

Binary Logistic Generalized Linear Mixed model in SPSS. Is it doable?

I want to analyses the proportion of swimming sperm of three species of fish in two salinities. To analyse the proportion of swimming sperm in a Generalized Linear Model, I would use a Binary...

03 March 2021 2,297 3 View

Looking for Materials with high thermal expansion coefficient and high temperature resistance?

03 March 2021 8,272 1 View

Guidelines for survey (question type) analysis?

Hi. Please tell me what guidelines should i need to follow for questionaries' type research work in India. It is not hospital based work, we are conduction basic institutional based qualitative...

03 March 2021 2,037 3 View

What are the values needed for gabor filter's parameter for cifar10 data?

Hi, I implemented a code to gabor filter cifar10 data but the images after being filtered and stacked are not clear like the original images. I think the problem is in the way I am using the...

03 March 2021 6,317 1 View

Can we block unwanted images during the x-ray prediction?

i am try to classify the x-ray images. During classification , can i block unwanted images (except x-ray image).

03 March 2021 7,100 1 View

No title

03 March 2021 5,360 2 View

What techniques can I use to determine the miscibility of polymer blends?

The term miscibility refers to the single-phase state in thermodynamics. I do not mean the compatibility of different components. To determine the miscibility I know several techniques such as...

03 March 2021 4,107 4 View

Sample dilution for quantitative analysis using ELISA?

If the detection range is in ng/ml but the reference range is in ug/ml for a molecule or protein in serum or plasma .how to dilute and what is the initial volume to be taken for quantitative analysis

02 March 2021 7,670 3 View

The procedure compute?

02 March 2021 5,204 3 View

In methylene blue test Why is UV absorbance reading very low as soon adsorbent is added and then increasing before reaching equilibrium?

Hello, I'm working on removal of methylene blue. 01. in the dark test very first reading is taken 15 minutes after adding adsorbent to the dye solution and that UV absorbance reading is very...

19 February 2021 472 5 View

How to raise temperature in small increments during equilibration in LAMMPS?

All, I am trying to equilibrate an Ice 1h crystalline structure to 250K in LAMMPS. When I tried to do this at 250K directly, the structure lost it's crystalline shape and became disordered post...

14 February 2021 5,747 2 View

How would I go about equilibrating the internal humidity of a closed system at 2 different temperatures?

I have a box which is kept at 4C which is enclosed in a ziploc bag. The dimensions of the box are: 18cm x 13.4cm x 4.9cm. The relative humidity of this internal environment is 30% at this...

07 January 2021 6,734 3 View

A problem with GROMACS 2020.3 ?

Greetings, I am trying to run GROMACS 2020.3 on a supercomputer using this command to do the production run: mpirun -np 24 gmx_mpi mdrun -v -s AB_md_0_1.tpr -deffnm AB_md_0_1 >...

17 December 2020 1,232 3 View

Kinetic modelling of oxidative dehydrogenation of ethane ?

According to below articles about kinetic modelling of ODH reactions by G. Che-Galicia and et al. there are some problems need your guidance and advices: Kinetic and reactor performance of a...

27 November 2020 9,035 3 View

How can i investigate the interaction between gas-rock or gas water rock in phreeqc?

I want to investigate the reaction between a mineral (Calcite) and a gas phase (H2) which has been stored at a depleted gas reservoir. I try to model this by defining a solution and put H2(g) as...

25 November 2020 8,916 1 View

Solve it given in description?

We consider a reaction system "A" reversibly reacts with "B" through k1 and k-1 rate constant respectively, and "B" gives "C" and "D" through k2 and k3 rate constant respectively through parallel...

27 October 2020 8,987 16 View

How to fix the problem about segmentation fault?

Hi everyone, I am new gromacs user. I am doing the MD simulation of protein-NA. I did the Energy minimization step, NVT, NPT equilibration. after equilibrating the system i submitted the...

21 August 2020 7,876 4 View

How to sure that a optimized structure is traped in a global potential well not in the local one for large systems?

Dear people, I have optimized a dimer (each monomer contains about 50 to 60 atoms) structure in its equilibrium distances (I have done a rigid scan to find the equilibrium distance between...

05 August 2020 7,683 1 View

Why does the equation of balance of Equilibrated force (equation containing voids) not include any thermal relaxation parameter?

In most mathematical problems considering voids, why does the equation of balance of Equilibrated force not include any thermal relaxation parameter considering Green-Lindsay theory or any higher...

20 July 2020 5,481 6 View