CD8+ T cells priming in vitro?

25 August 2023 0 9K Report

I have differentiated Cd8+ T cells from CD34+ cells isolated from cord blood. How can I prime them to become cytotoxic against gbm cell lines? Do I need dendritic cells? Is there a way to by pass them? How does Tall-104 cells kill cancer cells without priming? Can allogenic CD8+T cells be used to kill GBM cancer cell lines? Thank you.

Badges
Science topic

Similar topics
Social Psychology
Attitude

More Hema Mylammal's questions See All

How to get Equilibrium for Lennard-Jones Potential?

Hi all! I want to get equilibrium for Lennard-Jones potential. How it will find and implement our system. please suggest any idea.

10 March 2024 7,525 1 View

1. can we run isolated molecule (monomer dimer or trimer) as one unit only on gromacs?

Hi all, I want to run isolated molecules as one unit on gromacs. any idea how would i do that.

05 March 2024 2,096 2 View

Can we run MD without solvation?

Hello all, I had a question about MD, which I would appreciate if you could answer. I want to run MD without solvation. please suggest any idea.

05 March 2024 5,386 2 View

In BET analysis for surface area calculation do we need to consider pore diameter from the adsorption or desorption curve?

In addition to that question multipoint bet analysis will give us the total average surface area. Which one we should consider?

01 March 2024 416 3 View

What to do to get a good quality smoot PVDF film?

When I am trying to make PVDF film, after heating it seems to cracked and the white thick cast occured instead of glassy thin film. I am using 1gm pvdf with 8 ml DMF and 2 ml Acetone. And the...

18 May 2023 8,295 1 View

How to weight 5 millimolar of SrNo3 and 10 millimolar of FeNO3 in 15ml water?

Initial solution was prepared by dissolving 5 mmol of Sr(NO3)2 and 10 mmol of Fe(NO3)3·9H2O in 15 mL deionized water and the mixture was subjected to vigorous magnetic stirring to obtain a clear...

26 March 2023 8,558 0 View

How to functionalize the cerium oxide nanoparticles (CeO2) using APTES?

I have synthesized cerium oxide nanoparticles (CeO2 NPs) using the combustion method. My attempts to functionalize the NPs using the APTES have failed so far. After APTES functionalization, the...

07 November 2022 1,476 2 View

How to tilt a 3d geometry in HFSS by 45 degrees in a particular plane?

For example a square unit cell in xy plane to be tilted in the same plane by 45 degrees

23 May 2022 3,101 4 View

How to analyse this combine TG-DSC curve. I can found only one weightloss step?

How to analyse this combine TG-DSC curve. I can find only one weight loss step? how to interpret this graph. This is done for my ferrite sample calcinated at 600 degrees celsius.

19 November 2021 326 3 View

XRD score card 49 is Valid or Not ? how to find effect of doping in Xrd peaks ?

When I go through the XRD data match using Xpert highscore plus I got a file match of 49 scores for my undoped ferrite sample. Is it valid to my prepared sample. ? Also how could I find the effect...

09 November 2021 6,515 4 View

Why Do TDS and EC Increase with Larger Wastewater Volumes, While BOD and COD Decrease?

I have carried out MFC experiments on three different volumes, 50, 500 and 1000 mL of wastewater. Results after MFC treatment shows that TDS and EC are more in larger volumes of water i.e. TDS and...

09 August 2024 9,621 0 View

Could it be a cell culture contamination?

Hello everyone! I observed in my culture (htert-RPE1 cells) an orange- red particle at the bottom of the dish. It is visible to the naked eye as a very very small red dot. Could it be a...

09 August 2024 2,824 3 View

Is there any way to quantify bacterial and fungal cells in their mixed culture?

I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...

07 August 2024 7,535 4 View

How to normalize and take the significance of the MTT OD values with 3 replicates for the same cell-line?

Hi, I have a question about normalizing the MTT OD values for doing the statistical analysis. So, if we have 3 different plates and we call them 3 different replicates, so, first we would...

07 August 2024 8,106 4 View

Why activated CAR-Jurkat cell could not kill targets?

Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...

06 August 2024 641 2 View

Why do we equate male and female arousal?

Women, on the other hand, can become physically aroused (increased blood flow in the reproductive organs) without becoming psychologically aroused even in the slightest. (Robert Weiss)

05 August 2024 9,537 2 View

How to isolate lymphocytes from mouse spleen?

I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...

04 August 2024 9,913 7 View

How to Add Missing Water Molecules in Protein-Membrane Simulations?

I have protein-membrane simulations (PDB, PSF, DCD) and have noticed that water molecules near the protein are not visible in the simulations. How can I fix this issue? Is there a way to place the...

04 August 2024 1,200 2 View

Does long-term culture of murine Monocytes result in macrophage differentiation?

Hi, I am isolating monocytes from the bone marrow using the Mouse Monocyte EasySep kit. I want to treat these cells and monitor expression of specific markers over the course of 10 days. I will...

04 August 2024 7,282 2 View

To perform transfection with DharmaFECT Duo in AGS cells. Could you tell me what the ideal concentration is to avoid significant cytotoxicity?

I would like to perform transfection with the reagent DharmaFECT Duo (Horizon) on the AGS cell line. Could you please inform me of the optimal concentration to use without causing cytotoxicity in...

03 August 2024 3,851 1 View