How good are ELISA kits sold by EIAab?
We have a problem with bone marrow-derived dendritic cells. Has anyone experienced highly autofluorescent bone marrow-derived DCs and how have they dealt with this problem?
10 November 2013 2,640 5 View
Gene expression has two main processes; DNA to RNA called gene transcription, followed by RNA to protein called translation. When we perfom RT-PCR we use mRNA to look for genetic information...
02 March 2013 2,312 38 View
I want to measure IL-1beta, IL-4 and IL-12 produced by canine leukocytes by means of ELISA. Intracellular staining (FACS) appears not to be working well. I would appreciate suggestions on a...
08 September 2012 2,947 4 View
I am developing an ELISA kit for rotavirus A (G1P8 and G1P6). Does anyone know the weight (ng) of these two strains? Or to help me find their weight?
29 July 2024 3,138 2 View
hi, I am facing difficulties in the développement of an ELISA sandwich. I have several experiences in developing ELISA but that’s the first time I can’t find any solution. My test is currently as...
16 July 2024 9,044 10 View
I placed mice in metabolic cages to collect urine samples for analysis. However, the majority of the samples have dried up. Is there any way to retrieve the urine in those tubes without...
14 July 2024 6,724 0 View
Anyone have a protocol for making cell lysate from rat cardiomyocyte pellets to run an ELISA using PBS as the buffer?
23 June 2024 2,264 4 View
If cells obtained from different passages, with different plating but harvested on the same day are used in a single plate to take an ELISA measurement (with the given standards and treated...
17 June 2024 6,705 1 View
I used two standards (one came with the ELISA kit and the other I made myself, by preparing a series of known concentrations of the target analyte) on the same plate in my ELISA run. Why are the...
15 June 2024 5,109 1 View
Dear fellow researchers, We are conducting an ELISA with the "Cattle IGF 1 (Insulin-like growth factor) ELISA Kit" (Cat: ELK5630). Samples and reagents were prepared following the instruction...
13 June 2024 9,473 0 View
We have to report the concentration in pg/ml, the ELISA kit provider did not reveal the specific activity of the enzyme. In that case how to convert the units?
05 June 2024 2,173 5 View
I have a question about AUC for calculating SARS-CoV-2 neutralizing antibody tests. AUC here is not the Area Under the ROC Curve. After six serial dilutions of human serum, an indirect ELISA was...
05 June 2024 8,191 0 View
I want to measure a protein in serum using an ELISA kit. This kit allows serum samples to be diluted 200 to 3200 times, but I want to use this kit to titrate higher concentrations of protein. Can...
03 June 2024 4,937 0 View
