I want to evaluate inflammatory cytokines What will be more significant to study serum or tissue using ELISA?

09 September 2015 0 5K Report

I am studying cardiac remodeling and my tissue can have infarcted or non-infarcted portions.

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Science method

More Sulail Fatima's questions See All

I checked RNA conc. using nanodrop. Can anyone suggest what the peaks are speaking about?

I extracted RNA using Riboex (Geneall) followed by Trizol method. I have 2 types of tissues: Infarcted & non-infarcted cardiac tissue ( I am planning to perform RT-qPCR). I guess I am having some...

31 December 2017 7,866 17 View

Optimizing RT-qPCR for sybr green assay: Can anyone help?

I am optimizing RT-qPCR. I did RNA isolation from cardiac tissue using trizol method and cDNA synthesis using Takara cDNA synthesis kit. I have 13 pairs of primers and am optimizing assay for...

31 December 2017 7,117 12 View

Why no bands in gelatin zymogram? And something unusual at the bottom of gel?

I performed gelatin zymography on cardiac tissue extracts. I was expecting mmp-2 and mmp-9 bands as reported by references. Where did I go wrong? Both attachments are same gels. Just there is the...

31 December 2017 4,726 20 View

Which one I shall do first, qpcr efficiency and primer optimization?

It might sound lame but I am new to it. I don't get first I must optimize the primer or check for my qpcr efficiency by serially diluting my cdna. Can anyone briefly explain how I can do these...

11 December 2017 949 3 View

How much delta G is permissible for cross dimer, self dimer and hairpin?

I am designing primers for rt-qPCR and I am using premier biosoft to check the quality of my primers. Can I anyone suggest how much delta G is permissible for cross dimer, self dimer and...

06 July 2017 8,821 24 View

Enzymes for preparing single cell suspension from cardiac tissue for flow cytometry?

Can anyone suggest which enzymes I shall use for preparing single cell suspension from cardiac tissue. I will be looking at both cell surface and intracellular antigens involving cardiomyocytes...

06 July 2017 9,702 3 View

How can I prevent heart sections from being wrinkled?

After arresting the rat's heart diastole, I freeze it for an hour and half. I use rat heart slicer to make sections (2 mm) for ttc staining. After staining, my most of the sections are wrinkled. I...

11 December 2016 6,005 2 View

Why my SDS-PAGE gel is getting smeared?

I am studying MMP-2 and MMP-9 activity by gelatin zymography. The bands I have obtained are smeared up (within their lanes). Can anyone suggest possible reasons for this?

07 August 2016 5,328 10 View

Microtome tissue sectioning - How to prevent chatter of paraffin tissue specimen sections?

Hello everyone, I have recently started using the microtome device for sectioning of paraffin-embedded mice lung. While I had some success in sectioning and observing proper ribbons, some of my...

06 August 2024 666 4 View

Do you have good tips for seaweed tissue preservation in the field for post RNA extraction?

I will be with my students collecting seaweed samples in a marine farm and later we will process this tissue for RNA isolation and further sequencing. Does anyone have tips on how to collect the...

04 August 2024 501 2 View

How to Transform my handy-wavy explanation of why group I/II metals oxides in aqueous solution are basic into a rigorous argument?

I heard an explanation about something being a better proton acceptor or lone pair donor but that doesn't make sense. I couldn't explain in in terms of acid-base theory. The hand-waving way I saw...

03 August 2024 9,918 0 View

What is the problem with these tissue culture plants?

All plants are green but some of these plants becomes yellow. I did not found any reason. Please help me to find out the real problem.

01 August 2024 589 4 View

CHO-K1 suspension adaptation protocol?

I am trying to adapt adherent CHO-K1 cells in F12K+10%FBS to suspension culture in only F12K, but in vain. I have followed Mirus bio as well as Thermo protocols, please suggest any alternative....

31 July 2024 3,756 0 View

For the process of preparing decellularized matrices what is the ideal solution in which we can bring the tissue from slaughter house to lab?

I am planning to prepare a decellularized porcine tissue derived biomaterial. It would be very helpful if I get to know the composition of the solution which is ideal to bring the tissue sample...

31 July 2024 5,645 2 View

Can we use technical replicates for statistical analysis of multiplex cytokine data?

I have done an experiment using luminex technique, measuring 46 analytes. but my sample size is less (N=6, for case and control each). However, i have put up the experimental set in duplicates....

31 July 2024 3,665 3 View

How to prevent hemolysis after performing cardiac puncture to draw blood from mice in serum?

We have encountered an issue with the serum of mice being hemolyzed after the process of centrifugation. Could you please provide guidance to prevent hemolysis ?

29 July 2024 8,402 4 View

What is the weight of rotavirus A (ng)?

I am developing an ELISA kit for rotavirus A (G1P8 and G1P6). Does anyone know the weight (ng) of these two strains? Or to help me find their weight?

29 July 2024 3,138 2 View

Culture fibroblasts with or without FGF2?

I'm new to primary human fibroblast culture and I'm studying aging using cells from old and young donors. Some research groups have used media supplemented with FGF2, so I cultured cells in the...

29 July 2024 3,030 2 View