I checked RNA conc. using nanodrop. Can anyone suggest what the peaks are speaking about?

I extracted RNA using Riboex (Geneall) followed by Trizol method. I have 2 types of tissues: Infarcted & non-infarcted cardiac tissue ( I am planning to perform RT-qPCR). I guess I am having some...

31 December 2017 7,740 17 View

Optimizing RT-qPCR for sybr green assay: Can anyone help?

I am optimizing RT-qPCR. I did RNA isolation from cardiac tissue using trizol method and cDNA synthesis using Takara cDNA synthesis kit. I have 13 pairs of primers and am optimizing assay for...

31 December 2017 6,986 12 View

Why no bands in gelatin zymogram? And something unusual at the bottom of gel?

I performed gelatin zymography on cardiac tissue extracts. I was expecting mmp-2 and mmp-9 bands as reported by references. Where did I go wrong? Both attachments are same gels. Just there is the...

31 December 2017 4,599 20 View

Which one I shall do first, qpcr efficiency and primer optimization?

It might sound lame but I am new to it. I don't get first I must optimize the primer or check for my qpcr efficiency by serially diluting my cdna. Can anyone briefly explain how I can do these...

11 December 2017 834 3 View

How much delta G is permissible for cross dimer, self dimer and hairpin?

06 July 2017 8,428 24 View

Enzymes for preparing single cell suspension from cardiac tissue for flow cytometry?

06 July 2017 9,591 3 View

Why my SDS-PAGE gel is getting smeared?

07 August 2016 5,179 10 View

I want to evaluate inflammatory cytokines What will be more significant to study serum or tissue using ELISA?

I am studying cardiac remodeling and my tissue can have infarcted or non-infarcted portions.

08 September 2015 4,539 0 View

How can I measure MMP-1 protein activity or protein level from liver tissue?

Instead of checking only the mRNA level, I want to check the active protein level of MMP-1 in Liver tissue from mice. How can I do that?

03 March 2021 1,763 2 View

Does any one know how i can prepare liver tissue with transmission electron microscope with good fixation?

i have problem in preparation of liver tissue with bad cell organelles and how i can adjust osmolarity

03 March 2021 8,890 1 View

Does anyone know how to plot my station to a section plot on Ocean Data View (ODV)?

I made a vertical section plot with WOA .nc file on ODV, and now I want to plot my stations (csv. file) in it. Does anyone know how to import my points to the section? They are two different...

01 March 2021 3,610 1 View

Does anyone know an alternative product for cardiac Troponin T antibody for Immunofluorescence (Thermo # MA512960)?

We have been using Thermo Fisher anti-cardiac Troponin T antibody for immunofluorescence staining in FFPE mouse heart sections but it was recently discontinued. The literature shows it to be the...

01 March 2021 4,937 1 View

How to assign different material properties to each element in Abaqus ?

Hello, I am writing a python script to generate my model and write the input file. The problem is I have 1000 elements in my mesh and I want to assign different material properties to each...

01 March 2021 9,511 3 View

Interested to stain mice tissue with WGA 488 conjugate, anybody has a protocol to recommend?

Interested to stain mice brain tissue with WGA 488 conjugate, appreciate if anyone willing to offer me some guidance or suggestions! Thank you!

28 February 2021 3,951 2 View

Any specific pipeline or parameter to analyse total transcriptomics study of brain tissue samples?

I have two groups of brain samples, control and treated for example. It was total RNA nova seq sequencing. I tried all the available pipeline like: star+rsem+deseq2, Hista+stringtie+cuffdiff,...

27 February 2021 356 6 View

B-mercaptoethanol (BME) protocol?

Hi everybody, I have been running a multiplex IHC which allow me to stain the same tissue with different markers (Primary Ab + Secondary conjugated Ab) (AF487, AF555, AF647). Since I would like to...

27 February 2021 5,028 2 View

Looking for genomic DNA extraction kits from mouse tissue (centrifuge-based, not spin-column)?

Hello. I am looking to extract genomic DNA from mouse brain and lungs and I need a kit that is not spin-column based because I need to scale up the solution amounts for 400mg tissue (most cap at...

25 February 2021 155 2 View

Has anyone tried to scan fluorescence IHC slides with microarray scanner?

I have an old slide microarray scanner (VIDAR Revolution 4200) and would like to give it a "second life", as IHC scanner array (fluorescence dyes), FISH over tissue or similar. Has anyone tried...

24 February 2021 9,210 1 View