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Questions related from Sulail Fatima
I am optimizing RT-qPCR. I did RNA isolation from cardiac tissue using trizol method and cDNA synthesis using Takara cDNA synthesis kit. I have 13 pairs of primers and am optimizing assay for...
01 January 2018 7,060 12 View
I extracted RNA using Riboex (Geneall) followed by Trizol method. I have 2 types of tissues: Infarcted & non-infarcted cardiac tissue ( I am planning to perform RT-qPCR). I guess I am having some...
01 January 2018 7,806 17 View
I performed gelatin zymography on cardiac tissue extracts. I was expecting mmp-2 and mmp-9 bands as reported by references. Where did I go wrong? Both attachments are same gels. Just there is the...
01 January 2018 4,671 20 View
It might sound lame but I am new to it. I don't get first I must optimize the primer or check for my qpcr efficiency by serially diluting my cdna. Can anyone briefly explain how I can do these...
12 December 2017 894 3 View
I am designing primers for rt-qPCR and I am using premier biosoft to check the quality of my primers. Can I anyone suggest how much delta G is permissible for cross dimer, self dimer and...
07 July 2017 8,748 24 View
Can anyone suggest which enzymes I shall use for preparing single cell suspension from cardiac tissue. I will be looking at both cell surface and intracellular antigens involving cardiomyocytes...
07 July 2017 9,653 3 View
After arresting the rat's heart diastole, I freeze it for an hour and half. I use rat heart slicer to make sections (2 mm) for ttc staining. After staining, my most of the sections are wrinkled. I...
12 December 2016 5,960 2 View
I am studying MMP-2 and MMP-9 activity by gelatin zymography. The bands I have obtained are smeared up (within their lanes). Can anyone suggest possible reasons for this?
08 August 2016 5,264 10 View
I am studying cardiac remodeling and my tissue can have infarcted or non-infarcted portions.
09 September 2015 4,610 0 View
