None, I think

The most reliable and easy method would be flowcytometry. But you could also make cytospins, do an antibody fluorescent staining with the correct markers and count manually. It's a lot more labor intensive than doing flowcytometry of course.

You could perform colony assays to determine the proliferation and differentiation potential of the cells. We recently published an approach that allows you to determine whether the cells have myeloid, erythroid and/or megakaryocytic potential all in one assay. We affectionately refer to this as "ultracult."

(https://pubmed.ncbi.nlm.nih.gov/37271449/

Assay optimization for the objective quantification of human multilineage colony-forming units. . 2023 Aug;124:36-44.e3.Exp Hematol . doi: 10.1016/j.exphem.2023.05.007.)

Note that, while CFU assays provide the actual fate that the cells went down in vitro, they don't always reflect the actual potential of the cells.

Thank you Miss. Diane Krause