Does anybody look Erasmus K2 Projects Partnership?

Hello All, We are currently looking for Erasmus K2 Project partner in Europeen countries. Are there anyone interested in becoming a project partner in the social sciences and (or technical...

10 December 2019 6,491 2 View

Ethanol contamination during elution of cDNA with TE in RNA-seq library prep?

I am using NEBNext Ultra II RNA library prep kit for preparing libraries from bacterial samples. When the libraries ready, I did second Ampure bead purification for those have dimer peaks. During...

24 October 2018 8,971 2 View

Multi step ahead time series forecasting in SVR?

Hi, I am trying to do multi step ahead time series forecasting by using SVR. When I do forecast for training dataset, the results are pretty good. However, when I do iterative forecasting by...

24 July 2018 4,713 3 View

Which way is better for batch anaerobic reactor? Continuous nitrogen sparging or one time sparging?

I am trying to grow Bifidobacterium longum in a anaerobic reactor. I use MRS+ L-cysteine for the growth media (which is also regular growth media). I maintain anaerobic conditions with N2...

07 March 2018 9,449 7 View

How can i separate mouse embryo bone from tissue for RNA isolation, is there any easy solution instead of cutting the tissue?

I am trying to isolate RNA from bone to analyse gene expression levels in mouse embryo.

15 January 2018 3,918 2 View

Protein isolation and western blot from FFPE tissues?

we tried to isolate and western blot proteins from Formalin fixed paraffin embeded tissues. We did this protocol: 1. we got 5 x 10 micro meter paraffin sections. 2. xylene, 96-90-80-70 alchols,...

23 August 2017 9,064 3 View

I am using NEB 10-beta competent E.coli cells. Transformation is successful but colonies dont grow in LB. Do you have any advise?

I am using NEB PCR cloning Kit. There is pMiniT vector inside kit. I transformed ligation bwt my PCR product and pMiniT vector to NEB 10-beta competent E.coli cells. I used SOC growth medium for...

21 February 2016 2,608 7 View

Does anyone have any experience in ITK-SNAP for muscle DTI analysis and fiber tracking?

04 November 2014 1,459 3 View

Polymer composites suddenly solidifies?

I am making polymer composite by solvent mixing method. When I am trying to add filler solution in polymer solution it quickly get solidified. How to make them then uinform? It doesn't give time...

03 March 2021 7,540 4 View

What solvent can I used for Paal Knorr reaction between 6-amino hexanoic acid and 2.5 hexanedione?

I am try to make the Paal Knorr reaction between 2.5 hexnedione and 6-amino hexanoic acid, my problem is type of solvent to use, because 6-hexaminohexanoic acid is soluble in water but I am not...

02 March 2021 4,443 2 View

How to polymerize TEOS?

I want to polymerize TEOS on a surface. I know that NH4OH is a preferred catalyst and an alcoholic solvent should be used .But I can not find certain amounts of each material to use. Does any one...

02 March 2021 1,404 6 View

Does Napabucasin soluble in dichloromethane and in ethanol?

I need to prepare a solution of Napabucasin (inhibitor of cancer cell stemness) in dichloromethane. The drug is expensive, so maybe somebody already tried to do it. If it is soluble, what is the...

02 March 2021 9,945 3 View

How can extract ciprofloxacin hydrochloride disolve in water er to organic solvent such as dichloromethan or any other solvent?

I need a procedure to extract the ciprofloxacin hydrochloride from aqueous solution by using dichloromethane in detail,. I thank you very much.

01 March 2021 4,306 1 View

How to separate water from glycerol solvent in a solvent recovery vacuum distillation column?

I am currently doing my third year design project. I have been assigned to design a glycerol recovery column in vinyl acetate monomer production. The process unit before the recovery column is an...

01 March 2021 9,525 4 View

What is the state of the drug inside solid lipid nanoparticles (SLNs)?

I am a little confuse with the mechanism of solid lipid nanoparticles (SLNs). The main point of encapsulating drugs into SLNs is because some drugs have low solubility in water. Therefore, SLNs...

01 March 2021 3,703 3 View

How to make fractions of chloroform extract of plant, using seperating funnel?

28 February 2021 8,466 2 View

Why am i not getting solid after reacting APTES with salicylaldehyde?

The reaction of APTES with salicylaldehyde in ethanol leads to the formation of imine compound yellow solid which is mention in literature, but I am not getting yellow solid I don't know why? when...

28 February 2021 2,955 2 View

can I treat waste water containing solvents and alcohol with electrocoagulation?

there are solvents and alcohol in the waste water of a factory that produces disinfectants. Increasing this waste water requires very serious operations. Can I treat this waste water with...

27 February 2021 2,609 3 View