Hello,
When preadipocyte cells were seeded to differentiate and confluent 3 days later, detachment and peeling occurred in all wells when changing the culture medium.
60,000 for 12 wells. I am seeding 5,000 cells for cells and 96 wells. I've had this problem before.
The agents I use have not changed. I had this problem only in some passages. Wonder what it might be about. I pass the cells at 70%, without confluent.
thank you for your advice
hi again, can this problem be caused by refreshing with fbs medium while using fcs?
Hello Ezgi Nur Çil
Maybe cells are getting dislodged due to applying high pressure while changing the media or there is a low attachment on the 96 well plate due to the coating of plates.
Please try to differentiate the cells into 6 or 12-well plates. After complete differentiation, you can dislodge the cells and seed them into 96 well plates.
You can culture and differentiate them into FBS alone, no need to shift them into FCS.
I hope these points will help you.
All the best 👍
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