I want to create Amphiphilic Polysaccharide and PLA MPs by electrospray technique (single axial). Could you suggest suitable solvent ?

I know that PLA is soluble in DCM, not soluble in water and hard to dissolve in DMF. What kind of solvents will be suitable for electrospray system for the amphiphilic polysaccharide and...

13 October 2020 3,559 4 View

Does anybody look Erasmus K2 Projects Partnership?

Hello All, We are currently looking for Erasmus K2 Project partner in Europeen countries. Are there anyone interested in becoming a project partner in the social sciences and (or technical...

10 December 2019 6,491 2 View

Ethanol contamination during elution of cDNA with TE in RNA-seq library prep?

I am using NEBNext Ultra II RNA library prep kit for preparing libraries from bacterial samples. When the libraries ready, I did second Ampure bead purification for those have dimer peaks. During...

24 October 2018 8,971 2 View

Multi step ahead time series forecasting in SVR?

Hi, I am trying to do multi step ahead time series forecasting by using SVR. When I do forecast for training dataset, the results are pretty good. However, when I do iterative forecasting by...

24 July 2018 4,713 3 View

Which way is better for batch anaerobic reactor? Continuous nitrogen sparging or one time sparging?

I am trying to grow Bifidobacterium longum in a anaerobic reactor. I use MRS+ L-cysteine for the growth media (which is also regular growth media). I maintain anaerobic conditions with N2...

07 March 2018 9,449 7 View

How can i separate mouse embryo bone from tissue for RNA isolation, is there any easy solution instead of cutting the tissue?

I am trying to isolate RNA from bone to analyse gene expression levels in mouse embryo.

15 January 2018 3,918 2 View

Protein isolation and western blot from FFPE tissues?

we tried to isolate and western blot proteins from Formalin fixed paraffin embeded tissues. We did this protocol: 1. we got 5 x 10 micro meter paraffin sections. 2. xylene, 96-90-80-70 alchols,...

23 August 2017 9,064 3 View

I am using NEB 10-beta competent E.coli cells. Transformation is successful but colonies dont grow in LB. Do you have any advise?

I am using NEB PCR cloning Kit. There is pMiniT vector inside kit. I transformed ligation bwt my PCR product and pMiniT vector to NEB 10-beta competent E.coli cells. I used SOC growth medium for...

21 February 2016 2,608 7 View

Does anyone have any experience in ITK-SNAP for muscle DTI analysis and fiber tracking?

04 November 2014 1,459 3 View

How to compare two groups with only two measurements?

Hiiiii everyone! I have an enquiry on statistical analysis. I was looking for many forum and it's still cannot solve my problem. I want to compare means of two groups of data but only with two...

03 March 2021 8,796 3 View

Why does blocking with my immunogenic peptide cause nuclear fluorescence in ICC?

Hi all, I am validating a polyclonal antibody raised against human ANT4, through WB and ICC. In most of my controls, the antibody shows very little off-target binding, but when I pre-incubate the...

03 March 2021 5,999 3 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

What kind of fluid could I use for a pitfall trap that also does not invalidate molecular testing?

Hi, I want to start testing pitfall trap to obtain ants samples, but I need to conduct molecular analysis on those insects. So, what kind of fluid can I use? Ethanol expires too early and I need...

03 March 2021 5,978 5 View

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10?

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10? Since, the growth curve from day 2 to 10 won't be like the "Logistic curve" it might not follow logistic...

03 March 2021 1,401 3 View

Do we need to write protocol for a second study of a systematic review that has been registered and published?

I have conducted and published a systematic review and meta-analysis research with the topic related to public health and health pomotion (protocol was registed in PROSPERO). Now we want to...

03 March 2021 8,920 3 View

How to increase a model accuracy ?

dear community, my model is based feature extraction from non stationary signals using discrete Wavelet Transform and then using statistical features then machine learning classifiers in order to...

03 March 2021 6,994 5 View

Do I need to do linear regression before running mediation analysis using PROCESS MACRO by Hayes ?

I just wanted to check if I need to run a linear regression separately if I am using PROCESS MACRO to run mediation analysis. Thank you.

02 March 2021 4,359 3 View

Sample dilution for quantitative analysis using ELISA?

If the detection range is in ng/ml but the reference range is in ug/ml for a molecule or protein in serum or plasma .how to dilute and what is the initial volume to be taken for quantitative analysis

02 March 2021 7,670 3 View

Is There Any Feasible Method To Test Fluorescent Compounds Other Than Fluorescence Spectrometers ??

Is There Any Feasible Method To Test The Efficiency Of Fluorescent Compounds Other Than UV Spectrometers ? Suggestions Would Be Appreciated !

02 March 2021 5,785 3 View