I have recently changed the agar powder to a cheaper food grade one, the agar solidifies well and seems to be useful alternative, but i noticed recently few of the fungal cultures have more tendency to grow submerged hyphae, which grow within the agar medium rather than forming mycelium on the agar surface. Would it be the agar powder that caused the phenomenon?
Michael Richardson
Agar is agar, so I would not have thought so. The nutrients are in the medium, so that is where the mycelium would be expected to grow.
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Mariano Torres-Gomez
If you change the volume of the agar plate or the brand or nutrient concentration, you can be modifying the agar density. And also, if you change the strain.
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