Jeremiah Sia

4 Questions 19 Answers 0 Followers

Questions related from Jeremiah Sia

I noticed recently the fungal cultures tend to grow submerged hyphae instead of aerial in a solid culture medium, what would be the cause of it?

I have recently changed the agar powder to a cheaper food grade one, the agar solidifies well and seems to be useful alternative, but i noticed recently few of the fungal cultures have more...

15 May 2025 7,855 3 View

How to improve Sanger Sequencing?

Dear all, I am having problems with my Sanger sequencing results, the chromatograms are not looking good (please refer to the attachments for images and ab1 file). May I ask if anyone knows what...

27 December 2023 8,065 21 View

What's a fungal strain?

Hi, I am new to mycology, may I ask what's a fungal strain? how to identify a different fungal strain from the other? do i need to produce them in order to describe a species properly and how may...

17 December 2023 565 12 View

Fungal Mycelium DNA Extraction

Dear all, I am a rookie in mycology. I am trying to extract DNA from cultured mycelium. However, I have failed to obtain good concentration (less than 5 ng/uL from 50 uL elution mixture) and DNA...

01 January 1970 6,129 6 View