I need to prepare a PBS solution pH 7,2, to preserve nasal swabs after collecting nasal secretion. Can I use DEPC water instead of MiliQ?

Janaina Lustosa de Mello @Janaina-Lustosa-De-Mello

02 August 2016 1 3K Report

137mM NaCl

3mM KCl

8mM NA2HPO4

15mM K2H2PO4

autoclaved MilliQ water (here I would change to DEPC).

William Letter

What does your SOP specify? If water is used, a grade of water should be established (same for other chemicals).

"Milli-Q" water is a trademark (Millipore Corporation) for an ultrapure Type I or II grade of water (https://www.emdmillipore.com/US/en/product/Milli-Q-Integral-Water-Purification-System,MM_NF-C72876#specifications).

DEPC (diethylpyrocarbonate) is a chemical used to treat standard deionized water, not a type or grade of water.

Water should be specified by a minimum grade or Type, not by a brand name. Specifying the grade of water in a method or SOP would allow you to use any water that meets the established specification. If the DEPC treated water you have on-hand meets the same specifications and grade of the water you need, then it could be substituted, but why would you introduce a specialty chemical (DEPC) to your system when only ultrapure water is specified?

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