How to reconcile two sets of qPCR data from the same sample but acquired with different parameters (Ta, primer cc., etc.)?

17 November 2020 4,272 3 View

Sample size in G mellonella studies?

Hi everybody, I am working with G mellonella, and I know that one of the advantages of it is the lack of ethics commit and the 3R principles. However, I am very insecure about the sample size I...

29 October 2020 2,079 1 View

What is considered a good binding affinity energy value between macromolecules?

Hi everyone, Does exist any threshold for binding affinity value between macromolecules? E.g. Any value lower than -5.0 Kcal/Mol..... How to determine that the binding affinity among different...

22 September 2020 4,329 3 View

How to perform phase transformation modeling on ABAQUS?

Hello everyone, I am modeling an AM process called SLM, on which a laser scan a powder bed in order to build a part. I am currently having problem with the phase-transformation phenomena (from...

06 April 2017 7,623 4 View

Is there any protocol for defined media for macrophage cultures?

I want to perform an infectivity assay to test a metal compound and I need to guarantee that the medium hasn't it in a free form (apart from proteins). There is any protocol using a defined media...

19 January 2017 2,973 3 View

Should I be happy if my data gives me a null hypothesis?

I mean, if it contradicts the current literature it might be even more relevant than a positive hypothesis, but my subject in particular is already controversial. People everywhere are getting...

02 October 2016 3,052 5 View

Hello, I am using GAP43 (B-5) mAb and found 2 bands with around 50 and 39Kda in SH-SY5Y cells. Which one should I measure as a GAP43?

Antibody from Santa Cruz

07 August 2016 2,256 2 View

How do I teach Inertial Reference Frame on the High School Level: ?

12 October 2015 3,551 8 View

How can I compare my infected human sample results from western blot versus ELISA?

06 July 2015 1,081 5 View

Is there any good literature for Cost-Effectiveness Analysis in Health?

Is there any good literature for Cost-Effectiveness Analysis in Health (specially oncology and Rad Onc)?Suggestion of books or articles

14 December 2014 7,645 10 View

Computation of p-value of time series using linear fitting of highly seasonal data. Should I remove the seasonal components prior to computation?

I have a time series of monthly chlorophyll-a observations that spans from 1997 to present day. I have computed the p-value of the time series without removing the seasonality, and it doesn't...

19 May 2020 3,259 5 View

Why normalised fluorescence line height is so strange in most recent years?

I have been exploring data in the Agean sea from MODIS product normalized fluorescence line-height (nFLH). In many control areas, I have noticed a strange pattern on the fluorescence line-height...

01 February 2020 7,440 1 View

Help-me identify this genus of phytoplankton?

11 December 2019 7,761 4 View

Could someone tell me if this is phytoplacton, if so which?

11 December 2019 7,066 3 View

How can i extract the genomic material from micro-algal samples and microcystis strains?

I am working with some strains of cyanobacteria isolated from Zimbabwe's Manyame catchment - I want to extract their genomic material so that I may use it to confirm their identity. In my lab I...

23 October 2019 5,163 3 View

How can i extract the genomic material from micro-algal samples and microcystis strains?

I am working with some strains of cyanobacteria isolated from Zimbabwe's Manyame catchment - I want to extract their genomic material so that I may use it to confirm their identity. In my lab I...

09 October 2019 2,539 3 View

What could be a justification for using more than one biodiversity index?

For my project looking at the biodiversity of phytoplankton in the Arctic, I have calculated Hill numbers and Average Taxonomic Distinctness on my samples, and they both tell very different...

07 August 2019 3,029 3 View

What are the best R packages for flow cytometric data analysis?

I have flow cytometric data from a series of phytoplankton samples. I organized all the FCS files into flowSets using the flowCore package. My next step would be to determine compositional...

05 June 2019 6,609 3 View

What are the best R packages for flow cytometric data analysis?

I have flow cytometric data from a series of phytoplankton samples. I organized all the FCS files into flowSets using the flowCore package. My next step would be to determine compositional...

03 June 2019 1,499 3 View

Prevent phytoplankton growth?

Does anyone know how I can prevent a lake to be green? I think this is because of phytoplankton growing in water. This lake has some birds which drink from it and the answer must do not damage the...

04 May 2019 4,007 7 View