I will perform an experiment using shRNA HMGB1 from Sigma, but there is no instructions to resuspend the lyophilized product. Could someone help me?
Hello I am new to CRISPR-Cas9 and, for my project, I started to collaborate with another lab that claims expertise on this technique. The objective is to produce 3 cell lines, each with a...
25 March 2024 7,935 8 View
Hello I have been trying to solubilize oleic acid to add it in cell culture, as done in this article Insulin prevents fatty acid induced increase of adipocyte size "Oleic acid was prepared as...
02 October 2023 3,109 0 View
Can anyone tell me the gender of this phytoplankton? I drew it to make it easier. was found in the transposition of the eastern axis of the transposition of the São Francisco River from the...
27 September 2023 9,229 1 View
Hello everyone, I'm doing some research on the Reynolds functional groups, and I can't find any reference to some phytoplanktons, could you help me find the codon? Phomidium sp, Bracteacoccus sp...
20 September 2023 5,501 0 View
help me identify the gender of this filamentous microalgae
12 September 2023 1,214 0 View
In the photo we can see two objects, a larger one that I consider to be Microcystys wersenbergii and another smaller one that I can't know what it is, but if we look closely, it looks like it is...
12 September 2023 8,865 1 View
I am etching SiO2 to use as hardmaks to a diamond device. I've noticed that walls in diamond were not good due to the roughtness of the hardmas and now I have to improove the SiO2 etching process....
03 August 2023 442 2 View
Hello everybody. I need some help, I need the identification of the genus of this species. unfortunately, there are no measurements. It was found in the transposition of the São Francisco River.
28 March 2023 6,509 0 View
Hey guys. I need some help, I need the identification of the genus of this species. unfortunately, there are no measurements. It was found in the transposition of the São Francisco river.
28 March 2023 1,100 0 View
Hello I have a trinocular Olympus BX40 equipped with a very old low-resolution digital camera. I would like to adapt my Olympus SX50 HS in place of that camera. Is it possible? Does someone have...
03 November 2021 7,960 3 View
Hi! So i attempted to understand a novel protein behavior towards heat application by analyzing its secondary structure change. I subjected the protein to a thermal denaturation analysis using...
06 August 2024 1,989 3 View
Dear All, I am trying to transfect a pCDNA3.1 vector containing my gene of interest. The purpose is to figure out the localization of the protein of interest. I have fused the protein with GFP on...
31 July 2024 9,892 4 View
Hello , I established a stable cell line expressing GFP tagged to a centrosomal gene having G418 drug selection marker. I validated the stable line by IFA and Western blotting, results are fine....
29 July 2024 5,007 0 View
I want to do 2,3-butanediol dehydrogenase(BDH) enzyme purification to confirm its activity for 2,3-butanediol. Before that, I need to confirm which N or C terminal tagging is better for enzyme...
28 July 2024 366 3 View
I cannot confirm cell surface protein expression by flow cytometry even after transfection and antibiotic selection of cells. Does it take long for proteins to express on the cell surface? the...
28 July 2024 3,178 3 View
It has been long known that EDC carbodiimide and NHS easter's half life agains hydrolysis are highly related to pH. However, very surprisingly, not yet found a paper giving a table/chart of their...
25 July 2024 8,738 0 View
In running two-dimensional gel electrophoresis on bacterial protein, some spots that appear to match a protein sequence have a significantly more acidic isoelectric point than the calculated pI....
24 July 2024 8,076 3 View
I found that several expression constructs of published protein structures containing an N-teiminal fusion tag GAMGSGIQRPTST. What's the founction of this fusion tag?
23 July 2024 1,353 4 View
hi every one I am making vector construction (for fusion proteins) and in this moment I wanna to amplification of ADAM17 prodomain with PCR. to yet, I couldn't amplified the ADAM17 prodomain with...
21 July 2024 8,660 1 View
I need to know the approximate size of my target protein for Negative-stain EM. I have an alpha-fold predicted structure of the protein. I do not see any turtorial on internet for estimating the...
21 July 2024 5,732 3 View