How to reconcile two sets of qPCR data from the same sample but acquired with different parameters (Ta, primer cc., etc.)?

17 November 2020 4,272 3 View

Sample size in G mellonella studies?

Hi everybody, I am working with G mellonella, and I know that one of the advantages of it is the lack of ethics commit and the 3R principles. However, I am very insecure about the sample size I...

29 October 2020 2,079 1 View

What is considered a good binding affinity energy value between macromolecules?

Hi everyone, Does exist any threshold for binding affinity value between macromolecules? E.g. Any value lower than -5.0 Kcal/Mol..... How to determine that the binding affinity among different...

22 September 2020 4,329 3 View

How to perform phase transformation modeling on ABAQUS?

Hello everyone, I am modeling an AM process called SLM, on which a laser scan a powder bed in order to build a part. I am currently having problem with the phase-transformation phenomena (from...

06 April 2017 7,623 4 View

Is there any protocol for defined media for macrophage cultures?

I want to perform an infectivity assay to test a metal compound and I need to guarantee that the medium hasn't it in a free form (apart from proteins). There is any protocol using a defined media...

19 January 2017 2,973 3 View

Should I be happy if my data gives me a null hypothesis?

I mean, if it contradicts the current literature it might be even more relevant than a positive hypothesis, but my subject in particular is already controversial. People everywhere are getting...

02 October 2016 3,052 5 View

Hello, I am using GAP43 (B-5) mAb and found 2 bands with around 50 and 39Kda in SH-SY5Y cells. Which one should I measure as a GAP43?

Antibody from Santa Cruz

07 August 2016 2,256 2 View

How do I teach Inertial Reference Frame on the High School Level: ?

12 October 2015 3,551 8 View

How can I compare my infected human sample results from western blot versus ELISA?

06 July 2015 1,081 5 View

Is there any good literature for Cost-Effectiveness Analysis in Health?

Is there any good literature for Cost-Effectiveness Analysis in Health (specially oncology and Rad Onc)?Suggestion of books or articles

14 December 2014 7,645 10 View

Runs of nucleotides in Sanger sequencing?

I performed site directed mutagenesis, transformation, and then I sent out plasmids for Sanger sequencing and found out that there is extension of DNA just before the stop codon. I am not sure...

27 February 2021 547 3 View

I can detect my protein but the encoding DNA sequence has stop codons within. Should I ignore the stop codons?

I constructed a synthetic DNA library (scFv, VH-VL orientation) with a 3' reporter and 8x histag. I cloned and expressed this gene following which I performed an ELISA. The ELISA results suggested...

19 February 2021 7,006 5 View

Should I add a promoter upstream of my CRISPR-inserted gene?

Hello, I want to insert a gene into my cells using sgRNA, Cas9, and an HDR template. The gene that I will insert into has its own promoter. Because I will cut the gene downstream of its start...

14 January 2021 1,170 2 View

Does anybody have experience in expressing and incorporating two proteins (to express as a protein complex) in one cloning site?

I want to clone have two proteins cloned into pPROexHtb+ (which has one cloning site). I want to express them as a protein complex. My question is would you generally put a stop codon after first...

09 January 2021 8,811 2 View

Should i add ATG in the beginning of my polypetide sequence when i want to insert it in a phagemid?

Hi I want to make a recombinant m13 phage displaying my peptide as a fusion peptide on its surface pVIII. Should i add ATG at the beginning when i want to insert it in phagmid vector? Previously,...

18 December 2020 9,931 2 View

Do I need to add a start codon and stop codon for the sgRNA expression in crispr?

Dear All, I am now designing the sgRNA for knocking out the gene through crispr. Base on my learning experience, a start codon and a stop codon should be put in the N terminus and the C terminus...

16 December 2020 5,795 3 View

Can I modify beginning of pelB/ompA tag as described?

Hello all, I am currently trying to modify my plasmids so that it has a sequence coding the pelB or ompA tag in front of the proteins. The problem is that in order to paste such a sequence into...

25 November 2020 8,386 3 View

What is the possible biological explanation of the mutational pressure as a driving force on the evolution of particular species ?

In one analysis major role of mutation pressure was observed in codon usage bias of some species

19 October 2020 4,755 1 View

At what time points should pro-inflammatory cytokines levels be tested in a mouse model of Prototheca algae infection?

I'm working on establishing an murine infection model of the Prototheca algae. Not much is known about the dynamics of the infection, nor the induced immune response. I'd like to use the...

19 October 2020 2,908 2 View

Submission of dna sequences to NCBI?

When i submit dna sequences to NCBI, they ask for clarification saying "Some or all of the protein coding sequences contain internal stop codons, reading frame shifts (insertions/deletions...

03 July 2020 6,101 1 View