Hello everyone, I'm doing some research on the Reynolds functional groups, and I can't find any reference to some phytoplanktons, could you help me find the codon?
Phomidium sp, Bracteacoccus sp and Prototheca zopfii
Hello I am new to CRISPR-Cas9 and, for my project, I started to collaborate with another lab that claims expertise on this technique. The objective is to produce 3 cell lines, each with a...
25 March 2024 7,935 8 View
Hello I have been trying to solubilize oleic acid to add it in cell culture, as done in this article Insulin prevents fatty acid induced increase of adipocyte size "Oleic acid was prepared as...
02 October 2023 3,109 0 View
Can anyone tell me the gender of this phytoplankton? I drew it to make it easier. was found in the transposition of the eastern axis of the transposition of the São Francisco River from the...
27 September 2023 9,229 1 View
help me identify the gender of this filamentous microalgae
12 September 2023 1,214 0 View
In the photo we can see two objects, a larger one that I consider to be Microcystys wersenbergii and another smaller one that I can't know what it is, but if we look closely, it looks like it is...
12 September 2023 8,865 1 View
I am etching SiO2 to use as hardmaks to a diamond device. I've noticed that walls in diamond were not good due to the roughtness of the hardmas and now I have to improove the SiO2 etching process....
03 August 2023 442 2 View
Hello everybody. I need some help, I need the identification of the genus of this species. unfortunately, there are no measurements. It was found in the transposition of the São Francisco River.
28 March 2023 6,509 0 View
Hey guys. I need some help, I need the identification of the genus of this species. unfortunately, there are no measurements. It was found in the transposition of the São Francisco river.
28 March 2023 1,100 0 View
I will perform an experiment using shRNA HMGB1 from Sigma, but there is no instructions to resuspend the lyophilized product. Could someone help me?
23 May 2022 9,913 0 View
Hello I have a trinocular Olympus BX40 equipped with a very old low-resolution digital camera. I would like to adapt my Olympus SX50 HS in place of that camera. Is it possible? Does someone have...
03 November 2021 7,960 3 View
If I want to put mouse light chain(Sac1 and XbaI) and heavy chain (XhoI and SpeI) in pComb3XSS (phagemid) to form Fab, should I add stop codon at the light chain?
20 May 2024 806 0 View
For my project, I am attempting to purify an intrinsically disordered domain cloned in a pET28b vector. The strain used for overexpression is Rosetta II(DE3). Firstly, I encountered an unexpected...
23 April 2024 9,939 4 View
Hii all.. I have few sequences of Fusarium spp partial TEF region. I want to submit them to genebank to get accession numbers. Can someone please help how to submit sequences of coding regions? It...
23 April 2024 2,468 6 View
I am trying to submit sequences of COX-1 gene(mitochondrial gene) to gene bank. My sequences is almost 100% identical to a published one. It return a message saying that they contain stop codons...
12 April 2024 3,503 0 View
Hello, Does anyone knows how to get actual substitution matrices for codon ? I can get the amino-acid substitution matrices through the GiPhy website...
24 March 2024 5,614 0 View
I was doing site-directed mutagenesis using PCR. And I have ordered forward and reverse primers that are reverse complements of each other (both primers are around 45bp, 7 aa before and after my...
19 March 2024 5,082 0 View
I need to know that after codon optimization what should be the GC content?
04 March 2024 7,074 1 View
Hello, I am working on viral metagenomics and have started using the High Throughput Sequencing reads for virus genome assembly. Since, I am at the beginner level, I have some confusions. As these...
01 February 2024 296 1 View
Hello everyone, Is there anyone who has cloned with pAcGP67A secretion vector with GP67A signal peptide. I tried to clone with insert 1.2kb in the vector, MCS: BamHI and NotI. C-term Thrombin and...
12 December 2023 5,091 1 View
I am looking for vectors for expression of bacteriocin, and from pET series I think that 19b fits the most, I am just having few questions. When I clone my insert in NdeI and XhoI, i will have...
12 December 2023 2,321 5 View