I need to lyse the human osteoblasts on day 14 and 21 for ALP analysis can I use NaHCO3 , NaCO3 and Triton X 0.1 %. I am trying to find a published paper regarding this but struggling to find out. Only read it a thesis
" .......and a known volume of carbonate buffer solution (0.2M NaHCO3, 0.2M Na2CO3, 0.1% Triton X-100)..." what volume should I prepare this in ? 1 L. 50:50 ?