What is the impact of Washback in teaching a language or any other subject?

The importance of washback in teaching and learning the language through assessment

15 November 2020 3,559 4 View

How can we use 'assemblage theory' by multi-disciplines for designing a better children environment?

A research group is conducting a study across several cultures to investigate insights about issues in the environment of school-age children (6-12). The purpose is to explore practitioners'...

05 August 2020 1,967 5 View

Can you help in the two-minute survey about nightlife atmospheres in historical places?

19 July 2020 9,584 15 View

Load deflection curve in abaqus?

Ihahve modeled RC beam in abaqus, but the load decrease extremely after the maximum is reached, what could be the problem?

03 May 2020 2,566 8 View

What is the difference between connector type axial and friction in abaqus to define a bond between concrete and steel?

what is the best way to simulate the bond between steel and concrete if i will use connector, what is the difference between connector type axial and friction how i can define shear stress...

25 April 2020 5,880 3 View

Can I use anhydrous ammonium phosphate in oil and grease extraction?

hi, can I use anhydrous ammonium phosphate instead of anhydrous sodium sulfate in oil and grease extraction?

23 March 2020 9,987 3 View

Is test of significance(p value) necessary in some researchs??

hi, i'm writing a research about decolorization of waste water by fungi. the results obtained by % decolorization. Is it necessary to include test of significance??

29 December 2018 7,535 3 View

What's the typical active area for anode and cathode for EIS test?

EIS, Active areas of Anode, Active area of cathode, SOFC

16 November 2018 3,137 4 View

Can you help me ,i need resent researches on orthogonality in Banach Space?

I begin anew research on this topic

09 September 2018 9,062 5 View

How to count cells on micro carriers using trypan blue ?

I have cells attached on PLGA-MC in spinner flask and I want to get daily 1 ml sample of cells-MC in order to draw growth curve "proliferation" for my cells. the problem that I cant get the...

30 May 2018 9,135 1 View

Buffer to disrupt platelet alpha granules for PF4 quantification?

Hello! I want to quantify by ELISA the secreted (from platelets poor plasma) and the non-secreted (from platelet lysate) PF4 before and after TRAP stimulation. I will use the ELISA from R&D...

03 March 2021 1,499 2 View

Sample dilution for quantitative analysis using ELISA?

If the detection range is in ng/ml but the reference range is in ug/ml for a molecule or protein in serum or plasma .how to dilute and what is the initial volume to be taken for quantitative analysis

02 March 2021 7,670 3 View

Are protein samples in SDS compatible with ELISA?

I have used an AllPrep DNA/RNA/Protein Mini Kit QIAGEN kit to extract RNA and protein from my samples. At the end of the protein purification, I resuspend my protein in 5% SDS. Will these samples...

28 February 2021 7,370 3 View

Does anyone use antinuclear antibody (ANA) and anti-Phospholipid antibody ELISA kit for Non Human Primate serum samples?

I am looking for anti-nuclear antibody (ANA) and anti-Phospholipid antibody ELISA kit for Non Human Primate serum samples. Any suggestions highly appreciated. Thank you.

28 February 2021 6,512 3 View

Anyone know of a good progesterone antibody?

I am looking for a good progesterone antibody to quantify levels of progesterone in zebrafish. Applications would be ELISA, and immunohistochemistry.

28 February 2021 5,929 2 View

Benefits of the 24 welled plate?

We have always used the 96 welled plate, the 48 welled plate but apparently there is much evidence on the 24 welled plate and above all its benefits.It states it is the same you would use on ELISA...

28 February 2021 1,149 1 View

Standard curve shifting over time. How do I stabilise the ELISA?

24 February 2021 1,365 3 View

Can I use a protein-devoid condition as my negative control in ELISA?

Hello, I am running a sandwich-type ELISA where I am trying to inhibit a protein-protein interaction using small molecules. For each experiment, I determine my Z' (Z-prime) to determine the...

23 February 2021 7,429 1 View

Surprisingly low antibody titers against SASRS-COV-2 RBD in hamsters compared to mice?

21 February 2021 6,958 2 View

Can anyone advise me about a cortisol antibody made in rabbit to set up an ELISA?

I am trying to set up a cortisol EIA using mouse anti-rabbit IGg as coating and a rabbit cortisol antibody as secondary ab. I am searching for a sensitive and specific cortisol Ab made in rabbit

20 February 2021 3,366 5 View