I need the buffer of ph 7-14 for enzyme activity can you please send me the detail preparation procedure of the buffer.

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Can anybody guide me for How to solve polynomial equation for checkpoint analysis in formulation development"?

I have done formulation development of tablet for which i have considered two independent variable. Results are plotted in a statistical analysis software and polynomial equations are generated....

24 April 2024 3,951 1 View

Why plant taxonomist only follow APG system of classification. Why countries like India don't have their region based classification system ?

Ancient India believed in traditional system of schooling called Gurukul, however sociopolitical developments and modernization of culture gradually wiped away the tradition. This eventually...

20 April 2024 9,414 0 View

Do electrons really revolve in orbits in atoms?

https://youtu.be/etausaYnFqQ?si=hxtrW321C5N8JtTT

14 January 2024 3,914 0 View

How to avoid Pin holes and micro porosity issue in thermal Spray and Induction Fused coaitng?

•Coating method- Thermal Spray and Induction Fused. (Thermal coating by metco 6P-2 torch and fusing by induction heater.) •Part material- AISI 1035 Steel Plunger (4.5'' Dia.) coated by Water...

06 November 2023 7,445 1 View

Has it really been verified experimentally that there is no phase difference between electric and magnetic fields in an electromagnetic wave?

In an electromagnetic wave, the energy of the electric field is converted into a magnetic field and vice versa. So there should be a 90 degree phase difference between them. Not only this, as the...

26 July 2023 9,417 5 View

How would I calculate Specific capacity from CV and GCD of supercapacitive study?

As my material is showing battery-type behavior, so I want to calculate specific capacity ( C/g or mAh/g) instead of specific capacitance (F/g) in the case of CV and GCD. Please suggest.

04 April 2023 3,416 2 View

Do we really know, how the energy difference of an electron is converted into an electromagnetic wave with particular frequency?

Really we don’t know. We know only the frequency of the wave equals to that of energy difference divided by the plank’s constant. If any charge vibrates with a particular frequency,...

01 March 2023 5,183 6 View

What are the dimensions for the apparatus of novel object recognition test (NOR) used in rats? And what object pairs well for the test?

We are trying to make NOR for adult Wistar rats in our lab. The literature varies on the dimensions that should be used. I wanted to know what can work best for this. any kind of help is appreciated.

02 July 2022 4,352 2 View

Can we generate the scattering matrix and Coherence matrix for the dual-pol SAR?

I want to calculate the scattering matrix and the coherence matrix for the dual-polSAR is it possible to calculate? or calculation of the scattering matrix and the calculation of the coherence...

05 December 2021 3,992 9 View

Microspeaker: How to evaluate the Stiffness(K) & force factor (Bl) in polynomial coefficient's analytical form of a microspeaker?

I am looking for the polynomial coefficient's analytical form of 2nd and/or higher-order (ex: b0, b1, b2 ...in the force factor and k0, k1 k2... in stiffness). Most of the available works of...

10 August 2021 793 0 View

AMAXA Lonza nucleofection lower volume of buffer?

Does anyone tried to do nucleofection with AMAXA by Lonza with lower than recommended amount of buffer in the cuvettes (100 ul)?

07 August 2024 4,616 0 View

Why I can't see any band in SDS-PAGE?

Currently, when I run SDS-PAGE, I don't see any bands at all, even though I used the same material just a day ago and it worked fine.... In our lab, we dilute the 10X running buffer to 1X and...

06 August 2024 5,373 2 View

How to assess the osmotic power of a substance?

Hello, I suspect that a molecule I am using is causing bacteria to experience osmotic shock. What chemical properties should I look for to compare this capability with those of other substances?...

06 August 2024 977 1 View

Why might the impedance values for DI water and 0.1X PBS buffer solution exhibit a decreasing and increasing trend, respectively over time (HP 4194A)?

Hello everyone, I'm encountering an issue with my electrochemical impedance spectroscopy (EIS) measurements and would appreciate some insights. Experimental Setup: Electrodes: Gold interdigitated...

05 August 2024 3,783 2 View

What is the best blank for nanodrop if I want to read a recombinant protein concentration?

Is it the "elution buffer" or the "dialysis buffer"? Note: I'll be using NanoDrop OneC

01 August 2024 967 3 View

How to check pH of a high strength hydrogel?

We are working on biopolymeric hydrogels. Our system is highly viscous and sticky, and the gel formed are high in strength. We are unable to use pH electrode and pH strip. Please suggest an easy...

30 July 2024 942 2 View

EDTA buffer solution?

Hello everyone! How can i prepare EDTA buffer solution (pH 8.2) to determine GSH levels? Thank you in advance.

29 July 2024 4,374 1 View

Whether i can preserve leaves samples in 2.5% glutaraldehyde in 0.05 M phosphate buffer, pH 7 for coming several weeks for further LM study ?

I want to preserve the sample leaves for the LM study of the stomata and conidia analysis. Whether 2.5% glutaraldehyde in 0.05 M phosphate buffer with pH 7 can be applied for preservation.

29 July 2024 8,560 0 View

Can a shoot-through event of a tri-state digital buffer cause momentary Hi-Z state?

// interested in the difference between floating events and short circuits.

22 July 2024 6,565 0 View

How can EDTA be removed from already extracted DNA samples?

Our lab has extracted bacterial DNA samples using Qiagen DNeasy kit and eluted the samples in AE buffer. However, this buffer contains 0.5mM EDTA not suitable for downstream application. Any ideas...

13 July 2024 1,675 3 View