I need the buffer of ph 7-14 for enzyme activity can you please send me the detail preparation procedure of the buffer.

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Please tell me, which temp can I select for neglect black color of the Calcium oxide nanoparticles ..

14 January 2021 5,341 5 View

Why Porphyrin is purple in color?

Porphyrin solid is purple in color, what factors are responsible for purple color of the porphyrin?

29 November 2020 199 2 View

After successful docking, how you can decide whether ligand act as an activator or inhibitor?

The interaction between drug target and ligand (drug) was performed with the help of biotools or online server(autodock or swissdock). After successful docking, how you can predict desired...

14 February 2020 4,434 15 View

Cosine Similarity between columns of two dataframes of differing lengths ?

Hi All I have text column in df1 and text column in df2. The length of df2 will be always > length of df1. I want to calculare cosine similarity for every entry in df1[text] against every entry...

30 December 2019 7,453 1 View

How to determine if a white colored precipitate on the surface of an extruded aluminium sample (multi-port tube) is aluminium hydroxide?

Dear researchers, I have some multiport extruded tubes with a certain white precipitate visible on the iner side of the tubes and would like to know a way to determine what that precipiate...

10 November 2019 3,318 7 View

How to measure FWHM from XRD analysis?

From XRD analysis how to measure Full Width Half Maxima (FWHM)?

15 September 2019 4,589 5 View

Which Nano-particles have more heat carrying capacity?

Which Nano-materials have high fire point, flash point, thermal conductivity and heat transfer coefficient. So that it can be used in a coolant incorporated with base fluids.

18 March 2019 8,869 4 View

What would be the reason behind formation of the W/O emulsion instead of O/W emulsions at higher concentration of material dissolved in oil?

I am trying to make the Oil in Water emulsion. If I dissolve 1% my material in oil then it forms O/W emulsions. But when I use concentration of my material Above 5 % then it forms W/O...

23 January 2019 4,396 3 View

How SCADA is differ from PLC?

In PLC we can store and control the program but what the thing that differ in SCADA compare to PLC?

25 November 2018 5,339 7 View

No title

I have done GCMS of my compound. Basically its mixture of number of compounds. When I do the GCMS then I found the same compound detects two to three time at different retention time in single...

18 November 2018 6,808 2 View

Buffer to disrupt platelet alpha granules for PF4 quantification?

Hello! I want to quantify by ELISA the secreted (from platelets poor plasma) and the non-secreted (from platelet lysate) PF4 before and after TRAP stimulation. I will use the ELISA from R&D...

03 March 2021 1,499 2 View

Does the pressure(-600 mmHg) affects pH?

When I conducted the degassing experiment using decompression under anaerobic condition(and I used ferrous based chelating agent), the oxidized ferric were observerd. So, could you tell me the...

03 March 2021 4,892 5 View

Buffer Exchange using column packed with sephadex G-25 effect on protein concentration?

Hello, If i am doing a buffer exchange for an antibody of 1mg/mL, does the elution lose protein in the process of buffer exchange? For example, if i flow through 500 uL of 1mg/mL sample, and...

03 March 2021 6,299 3 View

How optimize the Size Exclusion Chromatography?

Hello! I'll do a size exclusion chromatography, but I only have an open column, and I'll perform the peptide extraction from yeast, using buffer lysis (sodium phosphate 50 mM/NaCl 30 mM/DNAse and...

01 March 2021 2,215 2 View

Why are my bands weird in this Agarose gel?

Can someone please give me some possible things that could go wrong? Here is my recipe: 0.5g Agarose 50 mL of TAE 1x 1 uL ethyl bromide. Gel was run at 100V for 1 hour. The buffer used is also TAE.

01 March 2021 9,952 3 View

What do I do with DLS peaks that are from the buffer?

I'm looking at the aggregation of my protein sample using DLS. Unfortunately, my buffer (20mM HEPES) also results in a set of peaks. These are at approximately 1 and 1000 d.nm. The lowest peak...

01 March 2021 9,015 2 View

Which database would give me this list of chemical components?

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26 February 2021 5,525 3 View

What is the most suitable resuspension buffer for TCA/acetone precipitated protein?

I need to extract protein from fermented carbon sources for Bradford assay. Most researchers experiencing insolubility of pellet in resuspension buffer. Please assist me to select most suitable...

26 February 2021 7,129 3 View

What urea buffer can i use for biotagged protein extraction from streptavidin beads?

Hi all, I have been doing research on biotagged LDB1 proteins in Mel cells. I purify the proteins using M280 streptavidin beads. The yield is very low so I've been trying to optimize it by...

24 February 2021 5,749 3 View

Why is NH4OH added in heavy metals limit test?

The limit test I'm referring to is USP method II. In this procedure you need to dropwise add 6N of NH4OH to the sample until it the pH is alkali. After that you need to dilute and add HOAc until...

23 February 2021 2,049 3 View