Do anyone have experience to use anti-PDGFRa antibody for FACS?

I previously used APC-PDGFRa (clone APAA5),eBioscience for FACS. At that time dilution, 1:50, and 1:100 worked very well. but nowadays it's not working. could anyone help to guide how it happens...

23 April 2020 4,053 8 View

Regarding the Postdoc opportunities the subject like Agricultural Extension?

24 February 2020 1,782 13 View

Any other chemical instead of e hydroquinone (HQ) which i can attached with hexamethylenetetramine (HMTA)?

23 March 2019 7,305 3 View

Why we add ammonium hydroxide to the polymer solution in EOR??

Enhanced Oil Recovery

31 May 2018 6,642 1 View

How neem (Azadirachta indica L.)oil coating be done on urea to increase nitrogen use efficiency?

I have to plan my research on increasing nitrogen use efficiency and mitgating nitrous gas emissions and ammonia volatilization losses and studying the behavior of nitrifying bacteria by coating...

12 November 2017 5,139 5 View

My previous PI won't submit my research paper it's been sitting on his desk for three years ago. What should I do?

The project was my idea, and I did the work independently. Since it wasn't my PI's idea, he's not excited about it. This is still a high-impact paper, and it's important to my career. I might be...

03 April 2017 8,874 3 View

Why all cells show orange color when stained with Acridine orange /Ethidium bromide under green flourescence?

07 April 2016 5,745 4 View

How to construct standard curve for human mitochondrial DNA by real time PCR?

Hi everbody. I have been studying human mitochondrial DNA and cell free nuclear DNA in whole blood using mutliplex real time PCR. I just can't make standard curve because I can't make plasmid as...

24 March 2016 8,135 3 View

Can Hall Effect (HMS-5000) measurement system be used for Nano-structures?

I have synthesized CdZnS semiconductor nano-wires on ITO substrates, is it possible to measure carrier concentration and mobility through Hall Effect HMS-5000 system because it assumes that thin...

29 October 2014 8,624 2 View

How can you study Machine translation online?

Machine translation is one of the oldest subfields of artificial intelligence research. However, real progress was much slower. The use of statistical machine translation systems has led to...

21 October 2014 3,874 7 View

What is the best way to keep an anaerobic chamber anoxic in the absence of hydrogen?

I'm a graduate student who works with an anaerobe, so I often work in an anaerobic chamber. I've been working on an assay that involves the steady-state of the quinone/ quinol pool. Anoxic...

03 March 2021 2,971 1 View

Is there any tutorial for comsol general projections?

Hi, im trying to make a projection in a 3d comsol model. I have a non uniforme chanel and my intention is to get the average tempreture in the y-z cross section and se the average tempreture...

02 March 2021 4,624 4 View

Good sites to recruit survey participants on a grad school student budget?

Any good recommendations for participant recruitment for online surveys on a grad school student budget?

02 March 2021 4,293 4 View

Sample dilution for quantitative analysis using ELISA?

If the detection range is in ng/ml but the reference range is in ug/ml for a molecule or protein in serum or plasma .how to dilute and what is the initial volume to be taken for quantitative analysis

02 March 2021 7,670 3 View

Is it possible to do piezoelectric coupling in ANSYS-APDL or COMSOL between tapered substrate and rectangular piezoelectric patch?

How to do Piezoelectric coupling in ANSYS-APDL or COMSOL between tapered substrate and rectangular piezoelectric patch? Piezoelectric coupling between the rectangular substrate and rectangular...

02 March 2021 9,833 3 View

Any research papers for extraction of nutrient deprivation on macroalgae? Analysed by HPLC-UV and GC/MS. Possibly from macroalgae genus Rhizoclonium?

I am doing my thesis on the nutrient deprivation on the macroalgae Rhizoclonium sp. I will be analysing the levels over a period of roughly 5 days by HPLC-UV and GC/MS. I will be using seawater as...

28 February 2021 6,477 1 View

How can i resolve the "final solutuin is not converged" error in Comsol for my heat transfer simulation??

I have been trying to simulate heat transfer problem in Comsol 5.5 for long time but it always throw an error stating: "Failed to find a solution. Maximum number of Newton iterations...

28 February 2021 8,626 3 View

Necessary tube lenght to cool Natural gas in a dry-ice cooling bath?

I have a tank which contains natural gas at 250 Bar and 293 Kelvin. I want to cool the gas in a dry-ice cooling bath to about 195 Kelvin. The gas will flow in a copper-tube through the bath with a...

27 February 2021 8,128 1 View

Exit velocity of natural gas from pressurized tank?

Hi Guys. I'm a mechanical engineer and have a bit trouble, would be very glad if you could help me with my questions. I have a pressurized tank which contains Natural Gas at 200 Bar and 293...

26 February 2021 6,253 3 View

Is there a recitation score bank system for fair evaluation and assessment of student recitation?

In actual and virtual class, even if there is rubric for recitation, students may still fail to receive fair grades or scores. Others are active in recitation and others are not or others may fail...

26 February 2021 8,387 3 View