What does it mean sequence in frame. How we do full read of sequence.
A full sequence read is the complete sequence of an insert without gaps or ambiguities, essential for accurate downstream analysis, especially in coding sequences. A sequence in frame refers to the reading frame in which DNA or RNA sequences are read, particularly in protein-coding genes. A sequence is considered "in frame" if it is divisible by three without any remainder. Maintaining the reading frame in an insert is crucial for protein-coding genes, as any frameshift mutations or insertions/deletions can disrupt it, leading to non-functional or truncated proteins.
Example: If you have a sequence with 9 nucleotides (e.g., "ATGGCCTAA"), it is in frame because 9 is evenly divisible by 3 (3 x 3). This means it can be read as three codons: "ATG," "GCC," and "TAA." The sequence is not disrupted, and the reading frame is maintained.
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