I made a lytic reagent for 3-part cell counter. How can I reduce the hemoglobin percentage?

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Does anybody know why the transverse vortex force exerted on a SCR decreases as the suction resistance factor gets a higher value?

The transverse vortex force and in line vortex force exerted on a SCR have the maximum value near and in the TDZ when the seabed in modeled nonlinear. As the suction resistence has a higher value...

20 October 2022 8,550 0 View

How can I remove PVA to get pure graphene from my foam ( graphene + PVA )?

I made graphene out of graphite the process involved PVA, as a result of that I couldn't get graphene powder instead I got the combination of graphene and PVA which is a foam. How can I remove...

19 October 2021 7,261 3 View

Abaqus Tosca Optimization : How Thermal Design Response can be defined?

No matter if I chose topology or shape optimization tasks (or whether they are condition-based or general) I can not see any thermally related variable to select as my design responses for heat...

09 June 2021 1,127 3 View

Citation on LaTeX: How to include apa package from the scratch, step by step?

Hello dear readers and researchers, I am a beginner user of Tex Maker. How can I use packages that run citation on LaTeX?

20 January 2021 5,835 3 View

How to allocate separate parts of a LaTex document to several distinct parts in a uniform file?

Hello dear readers and researchers, I have begun learning La-Tex and I want to divide the main file into several section in separate sub sections of a uniform La-Tex project (pardon me for that...

17 January 2021 5,866 1 View

How to solve the error "not solved" in pipe segment simulation with hysys?

Hi all, I have a problem with a simulation with hysys. there is a wellhead separator (2 phase separator) that the outlet gas and liquid (water+oil) of this separator is entered the separate pipes....

28 March 2020 934 4 View

How can I reduce/remove the cytotoxicity of methanol?

Hello everyone, If I want to have several concentrations of a drug by using methanol as a solvent, how can I reduce or remove the toxicity of methanol in the solution? And is it necessary to do...

13 February 2020 7,118 3 View

How do I fix and permeabilize KYSE-30 cells?

For Immunocytochemistry (ICC), I want to use 4% formaldehyde and Triton 100-X for fixing and permeabilizing KYSE-30 cells. I want to know about the amount of these materials. Is there any...

25 January 2019 6,020 8 View

Is there any option in Abaqus FEA for temperature coupling?

I wonder if there is an option, rather than Tie, in Abaqus CAE to constraint the temperature of a node (i.e. its 11th DOF) to be equal to the one of another during the whole simulation time. In...

28 November 2018 1,930 7 View

Can any one help me with my IMAC?

Hi all, I used to purify my protein (expressed using PEt vector in BL21 E. coli) at 40 mM imidazole. After a year, lots of impurities are being copurified with my sample with the same method....

17 October 2018 5,249 1 View

Why I can't see any band in SDS-PAGE?

Currently, when I run SDS-PAGE, I don't see any bands at all, even though I used the same material just a day ago and it worked fine.... In our lab, we dilute the 10X running buffer to 1X and...

06 August 2024 5,373 2 View

Can I multiplex a mouse monoclonal and rat primary for IHC (mouse brain tissue)?

Hi all, I was just wondering if anyone has experience with multiplexing a mouse monoclonal primary and a rat primary. I'm trying to multiplex by incubating them in the same well but was told by a...

06 August 2024 9,710 1 View

How to preform densitometry on SDS-page bands?

I ran a SDS-page of a bacterial lysate and I want to quantify protein concentration in a specific band. I was thinking of using a standards ladder or make some standards are different...

05 August 2024 9,805 3 View

Interested in a SCOPUS collaboration?

Hi RG family. My team and I are working on some SCOPUS publications and we need co-authors who are willing and capable of undertaking both qualitative and quantitative-based studies. The scope...

02 August 2024 7,843 0 View

Interested in a SCOPUS collaboration?

Hi RG family. My team and I are working on some SCOPUS publications and we need co-authors who are willing and capable of undertaking both qualitative and quantitative-based studies. The scope of...

02 August 2024 8,572 0 View

Does anyone have issues using Prepman Ultra reagent for MicroSeq ID bacterial, fungal and yeast sample preparation?

I have been attempting to extract DNA from Bacterial, Fungal and Yeast banked samples (>1e7 cells) using Prepman Ultra reagent and I seem to be struggling to obtain a sequence. Although the...

01 August 2024 2,079 0 View

Following click reaction in cell lysates, protein is immobile and remains at the top of the gel in SDS-PAGE?

I am using CuBr/THPTA for a click reaction in total cell lysates. I am facing issues with my protein sample in non-reducing SDS-PAGE where it's not migrating properly and most of it remains at the...

29 July 2024 950 4 View

Whether i can preserve leaves samples in 2.5% glutaraldehyde in 0.05 M phosphate buffer, pH 7 for coming several weeks for further LM study ?

I want to preserve the sample leaves for the LM study of the stomata and conidia analysis. Whether 2.5% glutaraldehyde in 0.05 M phosphate buffer with pH 7 can be applied for preservation.

29 July 2024 8,560 0 View

Addition of EDTA during the synthesis of copper nanoparticles to prevent it from being oxidized?

I've attempted to use this method to synthesize copper nanoparticles. Copper nanoparticles can be synthesised using a variety of precursor materials. CuSO4, distilled water, NaOH, and EDTA are...

28 July 2024 8,027 3 View

Why my IgG antibody looks fragmented on non-reducing SDS-PAGE?

I am performing IgG purification and I have to show my results on SDS-PAGE. I use 10% tris glycine gel and prepare the samples under non reducing conditions. I am new to antibodies and therefore...

23 July 2024 6,664 6 View