Citation on LaTeX: How to include apa package from the scratch, step by step?

Hello dear readers and researchers, I am a beginner user of Tex Maker. How can I use packages that run citation on LaTeX?

20 January 2021 5,605 3 View

How to allocate separate parts of a LaTex document to several distinct parts in a uniform file?

Hello dear readers and researchers, I have begun learning La-Tex and I want to divide the main file into several section in separate sub sections of a uniform La-Tex project (pardon me for that...

17 January 2021 5,559 1 View

How to solve the error "not solved" in pipe segment simulation with hysys?

Hi all, I have a problem with a simulation with hysys. there is a wellhead separator (2 phase separator) that the outlet gas and liquid (water+oil) of this separator is entered the separate pipes....

28 March 2020 538 4 View

How can I reduce/remove the cytotoxicity of methanol?

Hello everyone, If I want to have several concentrations of a drug by using methanol as a solvent, how can I reduce or remove the toxicity of methanol in the solution? And is it necessary to do...

13 February 2020 6,927 3 View

Is there any option in Abaqus FEA for temperature coupling?

I wonder if there is an option, rather than Tie, in Abaqus CAE to constraint the temperature of a node (i.e. its 11th DOF) to be equal to the one of another during the whole simulation time. In...

28 November 2018 1,700 7 View

Can any one help me with my IMAC?

Hi all, I used to purify my protein (expressed using PEt vector in BL21 E. coli) at 40 mM imidazole. After a year, lots of impurities are being copurified with my sample with the same method....

17 October 2018 5,074 1 View

Any comments, ideas or experiences about single crystals shattering while removing from the container for mounting?

I am growing crystals of an air/moisture sensitive compound in a glove box. During the sample preparation for single-crystal XRD crystals will be crushed upon removal from the glass...

10 August 2018 9,335 2 View

Thermal analysis of machining in Abaqus?

I am developing a Lagrangian 2D model. I made parts for both work piece and cutting tool with dedicated material proprieties for each one. I defined plastic and damage model. Then to get...

20 December 2016 9,116 3 View

How to achieve coefficients (intercept and slope) of random factor and R2 in linear mixed effect regression analysis using R (package lme4)?

02 November 2016 2,210 9 View

What are the recent constitutive models of nonlinear method for ground response analysis?

Hello dear engineers I would like to study about the newest and recent constitutive models for using in nonlinear soil deposit response analysis. How can I find them? is there any specific...

20 June 2016 3,328 1 View

What are some important techniques for protein induction and SDS-PAGE analysis?

Hello everyone. I am fairly new in protein chemistry field. I am trying to perform a protein induction hoping to purify my protein of interest using FPLC method. However, I am having trouble on...

03 March 2021 8,237 3 View

SDS page software for analysis?

I did sds page to determine the difference in protein expression between 3 types of vaccine , but i don't have scanner or densitometer available. . so i wonder if there is a software to analyze...

02 March 2021 6,270 3 View

How optimize the Size Exclusion Chromatography?

Hello! I'll do a size exclusion chromatography, but I only have an open column, and I'll perform the peptide extraction from yeast, using buffer lysis (sodium phosphate 50 mM/NaCl 30 mM/DNAse and...

01 March 2021 2,215 2 View

Precipitate after adding laemmli buffer to protein solution containing Potassium Acetate?

Hi, I am running a size exclusion chromatography experiment with a buffer containing Potassium Acetate as a salt. I analyse these fractions through SDS-PAGE. After boiling my SEC fractions in...

01 March 2021 2,622 3 View

Are protein samples in SDS compatible with ELISA?

I have used an AllPrep DNA/RNA/Protein Mini Kit QIAGEN kit to extract RNA and protein from my samples. At the end of the protein purification, I resuspend my protein in 5% SDS. Will these samples...

28 February 2021 7,370 3 View

How can I represent SDS-PAGE results for machine learning analysis?

I am trying to classify and analyze the results of an SDS-PAGE based array for bacterial detection using machine learning, but I have trouble finding the best way to represent the results with...

27 February 2021 9,176 3 View

Unknown debris sheets observed in cell culture flasks?

Hi, We culture neural progenitor cells in T175 culture flasks coated with PLO-Laminin. We have successfully grown these cells with our protocol for months. Recently, we observed large sheets...

25 February 2021 3,443 4 View

Why tick's salivary gland extract didn't run in the gel (SDS PAGE )??

Greetings, I want to make SDS PAGE for my sample (Tick salivery gland extract), I tried concentrations 10%, 12%. I also tried high molecular weight ladder. But the protein stopped at the top and...

25 February 2021 7,698 3 View

What is a good reagent for lentiviral plasmid transfection in HeLa cells?

I have been using Lipofectamine 2000 but was hoping there may be a better protocol or one for something like Lipofectamine PLUS reagent.

24 February 2021 1,633 4 View

What urea buffer can i use for biotagged protein extraction from streptavidin beads?

Hi all, I have been doing research on biotagged LDB1 proteins in Mel cells. I purify the proteins using M280 streptavidin beads. The yield is very low so I've been trying to optimize it by...

24 February 2021 5,749 3 View