At what step should one add internal standards in tissue sample for LC-MS?

Hi everyone, I'm doing metabolomics of placental tissue in my lab. I added 1ml of 80% MeOH along with 2 isotopic internal standards namely, phenylalanine and palmitic acid to my sample after...

18 January 2021 9,520 4 View

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26 July 2020 1,511 0 View

How to connect Lithium ion cells (modelled using 2RC equivalent circuit approach in simulink) in series and parallel?

I have modelled a lithium ion cell in simulink using the 2RC equivalent circuit approach. I want to connect multiple cells in series and parallel to form a complete battery pack. In the literature...

13 June 2020 4,107 4 View

What is the COVID-19 Test Kit market size and industry forecast ?

COVID-19 Impact Analysis on COVID-19 Test Kit Market report published by Value Market Research provides a detailed market analysis comprising market size, share, value, growth and trends for the...

04 May 2020 9,241 3 View

How to calculate the leaf area from a drawn outline of a leaf on graph paper ? means is there any easy software or any other method to calculate ?

I have drawn the out line of leaves on a graph paper, and i have like 120 pages of sheets each containing 5 graph on an av. so its actually laborious to calculate it manually. I thing MATLAB is...

23 January 2020 7,544 3 View

Do we need to perform a pilot study in qualitative research? if yes, how?

If semi structured questions are designed to collect the data, do the statements require a feasibility test? If necessary, what is the process?

26 November 2019 328 17 View

Can i use stress from the predefined fields to apply confinement pressure for my SMA confined concrete model?

i want to model SMA confined concrete for that i need to apply prestress for the recovery stress gemerate as confining pressure in hoops arround the cylinder.

24 January 2019 2,804 3 View

How long can GFP plasmid DNA withstand sonication without hampering its expression?

11 April 2017 2,179 3 View

What is the probable reason behind this??

We have synthesized vanadium sulfide nanoparticle (VS2) through a simple solvothermal method. Nyquist plot( impedence spectroscopy) shows that VS2 modified glassycarbon electrode (VS2/Nf/GCE)...

27 February 2017 4,393 3 View

Can anyone help with brain tissue protein solubilization in Lammelli buffer after precipitation?

I had homogenized protein in RIPA buffer but was facing problem in running SDS-PAGE gel. So I precipitated protein with 100% TCA , washed with Acetone and then tried to resolubilize protein in 2X...

09 April 2015 9,554 3 View

Is there any test to determine the cardiovascular risk of patients with obesity? from the perspective of obesity?

We found tests to determine cardiovascular risk taking into account lipid profile, blood pressure, age, history of diabetes, other laboratories. But when weight is involved, there is a paradox. I...

02 March 2021 6,036 1 View

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02 March 2021 3,060 3 View

What SPSS analysis should I run with one IV (with two levels) and two DVs?

Hi, I am trying to figure out which test would be most appropriate to run for the following question: Is there a difference in task blood pressure between smokers and non-smokers? There are two...

01 March 2021 8,235 2 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View

What could be some reasons why unstimulated monocytes are getting activated?

Im doing PBMC isolation -> CD14+ enrichment using magnetic beads -> stimulation setup. My negative control is just cells in cRPMI but they seem to get activated over and over again.

28 February 2021 7,883 3 View

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28 February 2021 9,936 2 View