At what step should one add internal standards in tissue sample for LC-MS?

Hi everyone, I'm doing metabolomics of placental tissue in my lab. I added 1ml of 80% MeOH along with 2 isotopic internal standards namely, phenylalanine and palmitic acid to my sample after...

18 January 2021 9,520 4 View

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26 July 2020 1,511 0 View

How to connect Lithium ion cells (modelled using 2RC equivalent circuit approach in simulink) in series and parallel?

I have modelled a lithium ion cell in simulink using the 2RC equivalent circuit approach. I want to connect multiple cells in series and parallel to form a complete battery pack. In the literature...

13 June 2020 4,107 4 View

What is the COVID-19 Test Kit market size and industry forecast ?

COVID-19 Impact Analysis on COVID-19 Test Kit Market report published by Value Market Research provides a detailed market analysis comprising market size, share, value, growth and trends for the...

04 May 2020 9,241 3 View

How to calculate the leaf area from a drawn outline of a leaf on graph paper ? means is there any easy software or any other method to calculate ?

I have drawn the out line of leaves on a graph paper, and i have like 120 pages of sheets each containing 5 graph on an av. so its actually laborious to calculate it manually. I thing MATLAB is...

23 January 2020 7,544 3 View

Do we need to perform a pilot study in qualitative research? if yes, how?

If semi structured questions are designed to collect the data, do the statements require a feasibility test? If necessary, what is the process?

26 November 2019 328 17 View

Can i use stress from the predefined fields to apply confinement pressure for my SMA confined concrete model?

i want to model SMA confined concrete for that i need to apply prestress for the recovery stress gemerate as confining pressure in hoops arround the cylinder.

24 January 2019 2,804 3 View

How long can GFP plasmid DNA withstand sonication without hampering its expression?

11 April 2017 2,179 3 View

What is the probable reason behind this??

We have synthesized vanadium sulfide nanoparticle (VS2) through a simple solvothermal method. Nyquist plot( impedence spectroscopy) shows that VS2 modified glassycarbon electrode (VS2/Nf/GCE)...

27 February 2017 4,393 3 View

Can anyone help with brain tissue protein solubilization in Lammelli buffer after precipitation?

I had homogenized protein in RIPA buffer but was facing problem in running SDS-PAGE gel. So I precipitated protein with 100% TCA , washed with Acetone and then tried to resolubilize protein in 2X...

09 April 2015 9,554 3 View

Does Napabucasin soluble in dichloromethane and in ethanol?

I need to prepare a solution of Napabucasin (inhibitor of cancer cell stemness) in dichloromethane. The drug is expensive, so maybe somebody already tried to do it. If it is soluble, what is the...

02 March 2021 9,945 3 View

What is the state of the drug inside solid lipid nanoparticles (SLNs)?

I am a little confuse with the mechanism of solid lipid nanoparticles (SLNs). The main point of encapsulating drugs into SLNs is because some drugs have low solubility in water. Therefore, SLNs...

01 March 2021 3,703 3 View

Do you kow of any database containing data on retention time, log P and polarizability for a large number of molecules?

Hi, I am a chemistry undergraduate doing my dissertation project on HPLC. In my project, I need to predict the HPLC retention time of 20 aromatic molecules, the calibration set. To do this I will...

24 February 2021 2,602 1 View

How to seperate PEG-4000 from aqueuse phase ?

Hello everyone , Please tell me if It possible to seperat POlyethelyn glycol -4000 from an aqueuse media by other ways rather heating . I have tried salting out by electrolyte solution to...

22 February 2021 7,285 5 View

How to analyse monosaccharide compositions from soluble fiber?

I have tried to hydrolyze the sample using 2 M TFA at 100C for 24, 48 h and 120C for 2 h. From these conditions, the HPLC results showed that peaks were not separated into monosaccharide. I found...

17 February 2021 5,230 1 View

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Hello. I ask for help - I need to get lithium tetraborate (soluble salt) from lithium carbonate and boric acid. In literature, everything seems to be simple - mix, ignite and - it's done. But it...

16 February 2021 9,523 2 View

Is There are any idea to achieve H NMR signals for swollen compound ??

the samples are not completely soluble - just swelled the solvents that's mean the sample may be partially soluble any suggestion, please

16 February 2021 8,801 3 View

Which non-coordinating solvent dissolves anhydrous Iron(II)chloride?

Hallo, i am looking for a non coordinating solvent that dissolves anhydrous FeCl2 for a synthesis in which i can not use solvents like thf, pyridin etc.. I already tried benzene (no solubility),...

11 February 2021 6,105 1 View

DMSO concentration in cell culture? Precipitating while PBS is added?

I am testing a compound on RAW 264.7. the compound is soluble in DMSO. but to ensure that final concentration of dmso is not more than 0.1%, we are first dissolving it in dmso and then diluting it...

11 February 2021 1,136 3 View

Solubility of PMP in solvent?

What is the maximum solubility limit of poly(4-methyl-1-pentene) in N,N, dimethylformamide? I gone through solubility parameter concept but I did not get the answer for maximum amount of polymer...

02 February 2021 947 3 View