I want to make a marker free expression vector, therefore, I am replacing the antibiotic resistance site of a vector with reporter genes, To achieve my target i have to get amplification of 14 kb DNA from that plasmid. Please suggest me poymerase from which company i should prefer to get accurate amplification with TA overhangs.
Hi
Roche expand PCR kit will be appropriate for such long PCR amplifications. Or you may go for similar polymerase from other companies
Dear Vikas,
For PCR of large size DNA fragment (say around 14 kb) routine Taq Polymerase may not work. You may opt for TaKaRa PrimeSTAR GXL DNA polymerase or High fidelity Taq polymerase from fermentas for your work, Also, you may need to optimize the PCR conditions.
All the best
Try to use KAPA enzymes. They have great enzymes for a wide range of situations.
- Badges
- Science topic