What mistake I did during Gibson assembly of 5 fragments, I did not found transformed colonies. Can anyone suggest me?

Dear all, I did assembly of of five different DNA fragments having length (A= 975 bp, B=950 bp, C= 748 bp, D=1022 bp, Vector backbone= 1790 bp). The concentrations of these gel purified DNA...

09 October 2017 3,356 7 View

How to design in frame cloning construct for two genes that I can get both individual functional proteins from a single polycistronic RNA?

05 June 2016 4,508 4 View

I have to amplify a DNA sequence of about 14 kb from a plasmid, please suggest me which Taq polymerase should be used to get proper amplification?

01 February 2016 7,230 4 View

Is it possible to clone the PCR product directly into an expression vector?

I want to express an enzyme in cyanobacteria. For that I amplified the coding sequence of this enzyme from another organism. Is it possible to clone this PCR product directly into an expression...

02 March 2014 7,545 20 View

Can anyone suggest me a manual protocol for DNA purification from agarose gel?

I am trying to elute a DNA band from agarose gel using the manual protocol; in this I cut the DNA band from the gel on the transilluminator (tried to expose to UV light for very short duration),...

31 December 2013 6,024 42 View

May i use the SALTS OF Ni2+ at the place of Ni2+ for inducing the promoter activity

I am using a inducible expression vector for expression of gene. The promoter in this vector will induce in the presence of 5 uM Ni2+. I have salts of Ni2+, so will nickel salt work?

07 August 2013 2,868 1 View

How do I discard the Agarose gel (containing ETBR) after electrophoresis?

Since ETBR is a carcinogenic chemical, what would be an appropriate way to discard the agarose gel containing ETBR?

07 August 2013 1,802 27 View

Can anyone suggest to me why I am not getting amplification of the gene?

I am trying to amplify a gene 1.2 kb by using the self-designed forward and reverse primers (by using OLIGOS SOFTWARE) having the Tm respectively of 61.3 C and 63.0 C. The isolated genomic DNA...

07 August 2013 8,755 28 View

Is it possible to develop intergeneric somatic hybrids between bacteria?

If both organism have the same type of cell wall, plasma membrane but different growth requirements and growth rate, one organism has doubling time 24 hours and another have 30 minutes, then is it...

06 July 2013 8,826 0 View

Suitable cloning vector for cloning gene construct of 4 kb in cyanobacteria

hi everyone can anyone help me that, which cloning vector will be more suitable for cloning the gene construct of about 4 kb in cyanobacteria

07 August 2011 3,787 1 View

No title

02 March 2021 3,060 3 View

What do you know about biotechnology art?

What do you know about biotechnology art?

02 March 2021 1,653 3 View

Which is the best place in the world for the plant biotechnology research?

I am searching for a good place for the Post Doc,

02 March 2021 4,053 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

How was your experience with Biomedical and Biotechnology Journal?

How was your publication experience with BBRJ in terms of peer review and reviewer comments. Did you find it fair and transparent ?

23 February 2021 3,810 6 View

Are the topics below viable research in plant biotechnology?

1. Chemically induced mutagenesis in seed and qPCR detection and amplification of desired trait. 2. Site-directed mutagenesis in qualitative traits of a multiline seed.

04 February 2021 9,069 3 View

Is receiving Invitation to submit a article from the journal is big deal ???

I have recently received a invitation from the journal "Current Pharmaceutical Bio-technology" to submit a research article. As i didn't received any invitation before. so, is it normal to to...

04 February 2021 4,596 23 View

Why is Fermented Plant Juice (FPJ) always diluted with water? Could it be diluted with other solvents such as rice water?

I am currently making a research paper with my classmates in Philippine Science High School and we are trying to find references that mixed Fermented Plant Juice with other solvents instead of...

17 January 2021 5,099 4 View

What steps should one take to address post-harvest disease problems in a supply chain?

If I were to receive an import of a new fruit species in the country and, upon arrival, the whole lot was diseased, what steps do I take to diagnose and solve the problem? Is there a general...

10 January 2021 1,649 7 View

Why negative controls also showing the specific bands of promotor and other regions (GUS, GFP etc.)?

Dear Researchers, I did a transformation of an empty vector (pCAMBIA1301/1302) into my passion fruit plants, for the optimization of the transformation protocol. After transformation; DNA...

06 January 2021 8,465 99 View