I am using a inducible expression vector for expression of gene. The promoter in this vector will induce in the presence of 5 uM Ni2+. I have salts of Ni2+, so will nickel salt work?
Is there any other way to get Ni2+ except as a salt?
Dear all, I did assembly of of five different DNA fragments having length (A= 975 bp, B=950 bp, C= 748 bp, D=1022 bp, Vector backbone= 1790 bp). The concentrations of these gel purified DNA...
09 October 2017 3,526 7 View
I am willing to design a suitable construct of two genes in a single reading frame, both of which could express with same efficiency under the regulation of a common promoter and RBS. I have to...
05 June 2016 4,626 4 View
I want to make a marker free expression vector, therefore, I am replacing the antibiotic resistance site of a vector with reporter genes, To achieve my target i have to get amplification of 14 kb...
01 February 2016 7,352 4 View
I want to express an enzyme in cyanobacteria. For that I amplified the coding sequence of this enzyme from another organism. Is it possible to clone this PCR product directly into an expression...
02 March 2014 7,676 20 View
I am trying to elute a DNA band from agarose gel using the manual protocol; in this I cut the DNA band from the gel on the transilluminator (tried to expose to UV light for very short duration),...
31 December 2013 6,160 42 View
Since ETBR is a carcinogenic chemical, what would be an appropriate way to discard the agarose gel containing ETBR?
07 August 2013 1,970 27 View
I am trying to amplify a gene 1.2 kb by using the self-designed forward and reverse primers (by using OLIGOS SOFTWARE) having the Tm respectively of 61.3 C and 63.0 C. The isolated genomic DNA...
07 August 2013 8,886 28 View
If both organism have the same type of cell wall, plasma membrane but different growth requirements and growth rate, one organism has doubling time 24 hours and another have 30 minutes, then is it...
06 July 2013 8,945 0 View
How can we develop the competency in filamentous organism like Nostoc and Anabaena spp.?
11 December 2012 6,322 3 View
hi everyone can anyone help me that, which cloning vector will be more suitable for cloning the gene construct of about 4 kb in cyanobacteria
07 August 2011 3,934 1 View
I'm very interested in biotechnology and medical research and I'd like to meet a professional here and talk about it together
28 July 2024 3,798 4 View
List down the various factors responsible and how does this effect the overall biogas production when it is related to Hydraulic retention time of the digester based on the feedstock?
27 July 2024 4,800 4 View
I am pleased to announce the CUSABIO Biotechnology Scholarships for Students 2024! This scholarship aims to support outstanding students pursuing studies in the field of biotechnology. Here are...
22 July 2024 5,790 1 View
I was wondering if it is possible to form a permanent open "ssDNA bubble" similar to a transcription bubble (>13 nucleotides) within E. coli. These criteria are important: 1. Open ssDNA...
07 July 2024 8,275 5 View
Give in detail with available literature and website links for submissions
27 June 2024 9,753 1 View
Hey everyone! I'm having difficulty marking the C4 complement factor in Western Blotting. Has anyone had contact with this antibody or made it in-house? I have already used Anti-C4β (D-12)...
17 June 2024 6,124 1 View
Generally for bacterial DNA isolation we use a fresh culture of 2-5 days old. But if we use an older culture say 10-15 days old will it have any impact on the DNA content and the DNA isolation...
13 May 2024 3,061 11 View
I was curious as to why modern soybean half-seed protocols use 1/10x strength B5 salts during the co-cultivation step rather than full strength B5 salts? Also, are the B5 vitamins added at full...
07 May 2024 3,924 0 View
I am following the standard method which is mentioned in Tomotake Kanki et al., 2009 research paper Monitoring mitophagy in yeast: The Om45-GFP processing assay . I am growing the cells in SD-HIS...
04 May 2024 6,463 0 View
How are these exploited in biotechnological applications?
07 April 2024 566 5 View