I am using a inducible expression vector for expression of gene. The promoter in this vector will induce in the presence of 5 uM Ni2+. I have salts of Ni2+, so will nickel salt work?
Is there any other way to get Ni2+ except as a salt?
Dear all, I did assembly of of five different DNA fragments having length (A= 975 bp, B=950 bp, C= 748 bp, D=1022 bp, Vector backbone= 1790 bp). The concentrations of these gel purified DNA...
09 October 2017 3,356 7 View
05 June 2016 4,508 4 View
01 February 2016 7,230 4 View
I want to express an enzyme in cyanobacteria. For that I amplified the coding sequence of this enzyme from another organism. Is it possible to clone this PCR product directly into an expression...
02 March 2014 7,545 20 View
I am trying to elute a DNA band from agarose gel using the manual protocol; in this I cut the DNA band from the gel on the transilluminator (tried to expose to UV light for very short duration),...
31 December 2013 6,024 42 View
Since ETBR is a carcinogenic chemical, what would be an appropriate way to discard the agarose gel containing ETBR?
07 August 2013 1,802 27 View
I am trying to amplify a gene 1.2 kb by using the self-designed forward and reverse primers (by using OLIGOS SOFTWARE) having the Tm respectively of 61.3 C and 63.0 C. The isolated genomic DNA...
07 August 2013 8,755 28 View
If both organism have the same type of cell wall, plasma membrane but different growth requirements and growth rate, one organism has doubling time 24 hours and another have 30 minutes, then is it...
06 July 2013 8,826 0 View
How can we develop the competency in filamentous organism like Nostoc and Anabaena spp.?
11 December 2012 6,191 3 View
hi everyone can anyone help me that, which cloning vector will be more suitable for cloning the gene construct of about 4 kb in cyanobacteria
07 August 2011 3,787 1 View
02 March 2021 3,060 3 View
What do you know about biotechnology art?
02 March 2021 1,653 3 View
I am searching for a good place for the Post Doc,
02 March 2021 4,053 3 View
Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...
01 March 2021 8,169 2 View
How was your publication experience with BBRJ in terms of peer review and reviewer comments. Did you find it fair and transparent ?
23 February 2021 3,810 6 View
1. Chemically induced mutagenesis in seed and qPCR detection and amplification of desired trait. 2. Site-directed mutagenesis in qualitative traits of a multiline seed.
04 February 2021 9,069 3 View
I have recently received a invitation from the journal "Current Pharmaceutical Bio-technology" to submit a research article. As i didn't received any invitation before. so, is it normal to to...
04 February 2021 4,596 23 View
I am currently making a research paper with my classmates in Philippine Science High School and we are trying to find references that mixed Fermented Plant Juice with other solvents instead of...
17 January 2021 5,099 4 View
If I were to receive an import of a new fruit species in the country and, upon arrival, the whole lot was diseased, what steps do I take to diagnose and solve the problem? Is there a general...
10 January 2021 1,649 7 View
Dear Researchers, I did a transformation of an empty vector (pCAMBIA1301/1302) into my passion fruit plants, for the optimization of the transformation protocol. After transformation; DNA...
06 January 2021 8,465 99 View