If both organism have the same type of cell wall, plasma membrane but different growth requirements and growth rate, one organism has doubling time 24 hours and another have 30 minutes, then is it possible to develop the somatic hybrids among them? What should be probability for survival for somatic hybrid ?
What should be media composition for hybrid ? The media component for one organism include minimal salts (ph 7.8) while for other organism yeast extract, beef extract, peptone and sodium chloride (ph 7.0).
Both are Gram negative bacteria, thus if anyone have standardized protocol for preperation of protoplasts from Gram negative bacteria, please write me.
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