What mistake I did during Gibson assembly of 5 fragments, I did not found transformed colonies. Can anyone suggest me?

Dear all, I did assembly of of five different DNA fragments having length (A= 975 bp, B=950 bp, C= 748 bp, D=1022 bp, Vector backbone= 1790 bp). The concentrations of these gel purified DNA...

09 October 2017 3,356 7 View

How to design in frame cloning construct for two genes that I can get both individual functional proteins from a single polycistronic RNA?

05 June 2016 4,508 4 View

I have to amplify a DNA sequence of about 14 kb from a plasmid, please suggest me which Taq polymerase should be used to get proper amplification?

01 February 2016 7,230 4 View

Is it possible to clone the PCR product directly into an expression vector?

I want to express an enzyme in cyanobacteria. For that I amplified the coding sequence of this enzyme from another organism. Is it possible to clone this PCR product directly into an expression...

02 March 2014 7,545 20 View

Can anyone suggest me a manual protocol for DNA purification from agarose gel?

I am trying to elute a DNA band from agarose gel using the manual protocol; in this I cut the DNA band from the gel on the transilluminator (tried to expose to UV light for very short duration),...

31 December 2013 6,024 42 View

May i use the SALTS OF Ni2+ at the place of Ni2+ for inducing the promoter activity

I am using a inducible expression vector for expression of gene. The promoter in this vector will induce in the presence of 5 uM Ni2+. I have salts of Ni2+, so will nickel salt work?

07 August 2013 2,868 1 View

How do I discard the Agarose gel (containing ETBR) after electrophoresis?

Since ETBR is a carcinogenic chemical, what would be an appropriate way to discard the agarose gel containing ETBR?

07 August 2013 1,802 27 View

Can anyone suggest to me why I am not getting amplification of the gene?

I am trying to amplify a gene 1.2 kb by using the self-designed forward and reverse primers (by using OLIGOS SOFTWARE) having the Tm respectively of 61.3 C and 63.0 C. The isolated genomic DNA...

07 August 2013 8,755 28 View

Is it possible to develop intergeneric somatic hybrids between bacteria?

If both organism have the same type of cell wall, plasma membrane but different growth requirements and growth rate, one organism has doubling time 24 hours and another have 30 minutes, then is it...

06 July 2013 8,826 0 View

How to develop the competency in filamentous organism?

How can we develop the competency in filamentous organism like Nostoc and Anabaena spp.?

11 December 2012 6,191 3 View

How to compare 2 samples recruited with different sampling methods?

If I want to compare characteristics of 2 groups of patients, one group is recruited from a random sample of the general public while another group is from a sample with a fixed gender (male to...

26 February 2021 3,526 3 View

Do you consider undergoing a structure literature review / Meta analysis and getting numerical findings sort of quantitative method?

I am looking for a classification of the research methods included in literature review papers or Meta analysis ones .

22 February 2021 1,131 4 View

Can I do two kinds of mixed method in explanatory research?

Dear distinguish professors and scholars, I have a research in measuring two kinds: think tank capacity and research impact on policy. Can I do two kinds of mixed methods in order to find, "Is...

20 February 2021 6,897 1 View

How to analyse a (perceptual) study using differing/comparable sets of stimuli?

I plan on running an online perceptual experiment that has, let's say, 100 unique video stimuli altogether. Since these are too many to be rated by every participants, I would like to present the...

15 February 2021 7,444 2 View

How to analyse a split-plot design in stata ?

I have run an split-plot design experiment evaluating the efficacy of a treatment (with 3 whole plots and 4 sub-plots), and I am planning to analyse the collected data in Stata. However, I do...

14 February 2021 7,800 7 View

Can I use a likert survey for criterion sampling in a qualitative study or would that make it mixed methods?

I am doing a phenomenographic study on the reading experiences of avid v. Neutral v. Non-Readers. If I use a likert survey to criterion sample for participants, does that make it a mixed method...

09 February 2021 8,594 3 View

How to create the context-mechanism-outcome configuration (CMOc) in the realist methodology?

In the realist methodology (especially by Raymond Pawson et al), the CMOc is a common structure to explain what works, for whom, in what circumstances, and why? I'm always asked about the...

08 February 2021 2,792 1 View

Recommendations for sample size in visual qualitative research (using children's drawings)?

Hi everyone! Asking for a little help, if I may. I am working on a research project, where I aim to analyse children's drawings about Covid-19. I am fairly new to using visual data in qualitative...

07 February 2021 9,530 3 View

How do I create a categorical variable, level of exposure, and a dummy dichotomous, yes-no, variable for the Harvard Trauma Questionnaire Part 1?

I am using the Harvard Trauma Questionnaire (HTQ) Trauma Events scale (original version) that has 17 items (17 different traumatic events) with 4 response options, indicating 4 levels of exposure:...

04 February 2021 1,509 3 View

How to report path analysis / SEM with Yuan-Bentler correction from R output?

Dear all, I am currently writing my master thesis where I am analysizing my data using path analysis and SEM in R (lavaan package). Now that I like to write up my results, I am struggling with...

04 February 2021 8,993 2 View